論文 - 詳細
| RRC ID | 75966 |
|---|---|
| 著者 | Harrell K, Day M, Smolikove S. |
| タイトル | Recruitment of MRE-11 to complex DNA damage is modulated by meiosis-specific chromosome organization. |
| ジャーナル | Mutat Res |
| Abstract |
DNA double-strand breaks (DSBs) are one of the most dangerous assaults on the genome, and yet their natural and programmed production are inherent to life. When DSBs arise close together they are particularly deleterious, and their repair may require an altered form of the DNA damage response. Our understanding of how clustered DSBs are repaired in the germline is unknown. Using laser microirradiation, we examine early events in the repair of clustered DSBs in germ cells within Caenorhabditis elegans. We use precise temporal resolution to show how the recruitment of MRE-11 to complex damage is regulated, and that clustered DNA damage can recruit proteins from various repair pathways. Abrogation of non-homologous end joining or COM-1 attenuates the recruitment of MRE-11 through distinct mechanisms. The synaptonemal complex plays both positive and negative regulatory roles in these mutant contexts. These findings indicate that MRE-11 is regulated by modifying its accessibility to chromosomes. |
| 巻・号 | 822 |
| ページ | 111743 |
| 公開日 | 2021-1-1 |
| DOI | 10.1016/j.mrfmmm.2021.111743 |
| PII | S0027-5107(21)00006-3 |
| PMID | 33975127 |
| PMC | PMC8176624 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Chromosomes* / genetics Chromosomes* / metabolism DNA Breaks, Double-Stranded* DNA End-Joining Repair* DNA, Helminth* / genetics DNA, Helminth* / metabolism Meiosis* |
| リソース情報 | |
| 線虫 | tm1524 |