RRC ID 76102
Author Yuval O, Iosilevskii Y, Meledin A, Podbilewicz B, Shemesh T.
Title Neuron tracing and quantitative analyses of dendritic architecture reveal symmetrical three-way-junctions and phenotypes of git-1 in C. elegans.
Journal PLoS Comput Biol
Abstract Complex dendritic trees are a distinctive feature of neurons. Alterations to dendritic morphology are associated with developmental, behavioral and neurodegenerative changes. The highly-arborized PVD neuron of C. elegans serves as a model to study dendritic patterning; however, quantitative, objective and automated analyses of PVD morphology are missing. Here, we present a method for neuronal feature extraction, based on deep-learning and fitting algorithms. The extracted neuronal architecture is represented by a database of structural elements for abstracted analysis. We obtain excellent automatic tracing of PVD trees and uncover that dendritic junctions are unevenly distributed. Surprisingly, these junctions are three-way-symmetrical on average, while dendritic processes are arranged orthogonally. We quantify the effect of mutation in git-1, a regulator of dendritic spine formation, on PVD morphology and discover a localized reduction in junctions. Our findings shed new light on PVD architecture, demonstrating the effectiveness of our objective analyses of dendritic morphology and suggest molecular control mechanisms.
Volume 17(7)
Pages e1009185
Published 2021-7-1
DOI 10.1371/journal.pcbi.1009185
PMID 34280180
PMC PMC8321406
MeSH Algorithms Animals Behavior, Animal / physiology Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / genetics Carrier Proteins / metabolism* Computational Biology Dendrites / genetics Dendrites / metabolism* Dendrites / ultrastructure Models, Neurological Mutation Neural Networks, Computer Neurogenesis / genetics Neurogenesis / physiology Neuronal Plasticity / genetics Neuronal Plasticity / physiology Neurons / metabolism Neurons / ultrastructure Phenotype
C.elegans tm1962