論文 - 詳細
| RRC ID | 77270 |
|---|---|
| 著者 | Homma K, Niino Y, Hotta K, Oka K. |
| タイトル | Ca(2+) influx through P2X receptors induces actin cytoskeleton reorganization by the formation of cofilin rods in neurites. |
| ジャーナル | Mol Cell Neurosci |
| Abstract |
In physiological and pathological events, extracellular ATP plays an important role by controlling several types of purinergic receptors and changing cytoskeleton dynamics. To know the process of ATP-dependent cytoskeleton remodeling, we focused on cofilin, a key regulator of actin cytoskeleton, and investigated the dynamics of cofilin in PC12 cells through fluorescent protein-labeled cofilin and actin, Ca(2+) imaging, and fluorescence resonance energy transfer (FRET) techniques. As a result, ATP induced intracellular Ca(2+) increase, following cofilin rods' formation. ATP-induced cofilin rods' formation was not observed in cells expressing unphosphorylatable variant of cofilin. A P2X receptor agonist, but not P2Y, induced the formation of cofilin rods, whereas calmodulin and calcineurin inhibitors suppressed it. These results indicate that Ca(2+) influx through P2X receptors induces the formation of cofilin rods via calcineurin-dependent dephosphorylation of cofilin. This pathway might be one candidate to explain the effects of ATP on neuronal development and injury. |
| 巻・号 | 37(2) |
| ページ | 261-70 |
| 公開日 | 2008-2-1 |
| DOI | 10.1016/j.mcn.2007.10.001 |
| PII | S1044-7431(07)00234-5 |
| PMID | 17993279 |
| MeSH | Actin Cytoskeleton / drug effects Actin Cytoskeleton / metabolism* Actin Cytoskeleton / ultrastructure Actins / drug effects Actins / metabolism Actins / ultrastructure Adenosine Triphosphate / metabolism Adenosine Triphosphate / pharmacology Animals Calcineurin / metabolism Calcineurin Inhibitors Calcium / metabolism* Calcium Signaling / drug effects Calcium Signaling / physiology* Cofilin 1 / metabolism* Fluorescence Resonance Energy Transfer Fluorescent Antibody Technique Neurites / drug effects Neurites / metabolism* Neurites / ultrastructure PC12 Cells Phosphorylation / drug effects Rats Receptors, Purinergic P2 / drug effects Receptors, Purinergic P2 / metabolism* Receptors, Purinergic P2X Signal Transduction / drug effects Signal Transduction / physiology Up-Regulation / drug effects Up-Regulation / physiology |
| IF | 3.182 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |