論文 - 詳細
| RRC ID | 77358 |
|---|---|
| 著者 | Mace EM, Monkley SJ, Critchley DR, Takei F. |
| タイトル | A dual role for talin in NK cell cytotoxicity: activation of LFA-1-mediated cell adhesion and polarization of NK cells. |
| ジャーナル | J Immunol |
| Abstract |
LFA-1 is critical for NK cell cytotoxicity because it mediates adhesion of NK cells to target cells. Talin is thought to associate with the cytoplasmic tail of LFA-1 and activates its ligand-binding function. In this study, we report that talin is also required for LFA-1-mediated outside-in signaling leading to NK cell polarization. NK cells generated from talin1-deficient murine embryonic stem cells are defective in LFA-1-mediated adhesion. Although exogenously added manganese activates LFA-1 on talin-deficient NK cells and induces conjugate formation with target cells, their LFA-1-dependent cytotoxicity is impaired. Binding of ICAM-1-coated beads to wild-type NK cells induces reorganization of the actin cytoskeleton and coligation of the activating receptor NKG2D induces polarization of cytotoxic granules, whereas talin1-deficient NK cells fail to polarize with or without NKG2D coligation. Thus, talin1 plays a dual role in NK cell cytotoxicity, first by activation of LFA-1-mediated adhesion and then via LFA-1-induced NK cell polarization. |
| 巻・号 | 182(2) |
| ページ | 948-56 |
| 公開日 | 2009-1-15 |
| DOI | 10.4049/jimmunol.182.2.948 |
| PII | 182/2/948 |
| PMID | 19124737 |
| MeSH | Actins / metabolism Animals Cell Adhesion / genetics Cell Adhesion / immunology Cell Line, Tumor Cells, Cultured Cytotoxicity, Immunologic* / genetics Intercellular Adhesion Molecule-1 / metabolism Killer Cells, Natural / cytology Killer Cells, Natural / immunology* Killer Cells, Natural / metabolism* L Cells Lymphocyte Activation / genetics Lymphocyte Activation / immunology Lymphocyte Function-Associated Antigen-1 / physiology* Mice Mice, Inbred C57BL Mice, Knockout Signal Transduction / genetics Signal Transduction / immunology Talin / deficiency Talin / genetics Talin / physiology* |
| IF | 4.886 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | OP9(RCB1124) |