RRC ID 77367
Author Shin HW, Kobayashi H, Kitamura M, Waguri S, Suganuma T, Uchiyama Y, Nakayama K.
Title Roles of ARFRP1 (ADP-ribosylation factor-related protein 1) in post-Golgi membrane trafficking.
Journal J Cell Sci
Abstract ADP-ribosylation factor (ARF)-related protein 1 (ARFRP1) is a small GTPase with significant similarity to the ARF family. However, little is known about the function of ARFRP1 in mammalian cells, although knockout mice of its gene are embryonic lethal. In the present study, we demonstrate that ARFRP1 is associated mainly with the trans-Golgi compartment and the trans-Golgi network (TGN) and is an essential regulatory factor for targeting of Arl1 and GRIP domain-containing proteins, golgin-97 and golgin-245, onto Golgi membranes. Furthermore, we show that, in concert with Arl1 and GRIP proteins, ARFRP1 is implicated in the Golgi-to-plasma membrane transport of the vesicular stomatitis virus G protein as well as in the retrograde transport of TGN38 and Shiga toxin from endosomes to the TGN.
Volume 118(Pt 17)
Pages 4039-48
Published 2005-9-1
DOI 10.1242/jcs.02524
PII 118/17/4039
PMID 16129887
MeSH ADP-Ribosylation Factors / genetics ADP-Ribosylation Factors / metabolism Animals Autoantigens / metabolism Biomarkers / metabolism COUP Transcription Factor II / genetics COUP Transcription Factor II / metabolism* Cell Membrane / metabolism Endosomes / metabolism Golgi Apparatus / metabolism* Golgi Apparatus / ultrastructure Golgi Matrix Proteins Guanosine Diphosphate / metabolism HeLa Cells Humans Membrane Glycoproteins / genetics Membrane Glycoproteins / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism Mice Protein Transport / physiology* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Viral Envelope Proteins / genetics Viral Envelope Proteins / metabolism trans-Golgi Network / metabolism* trans-Golgi Network / ultrastructure
IF 4.573
DNA material pcDNA3-hARFRP1(WT)-HA (RDB20094) pcDNA3-hARFRP1(Q79L)-HA (RDB20095) pcDNA3-hARFRP1(T31N)-HA (RDB20096)