RRC ID 78674
Author Li R, Kato H, Fumimoto C, Nakamura Y, Yoshimura K, Minagawa E, Omatsu K, Ogata C, Taguchi Y, Umeda M.
Title Essential Amino Acid Starvation-Induced Oxidative Stress Causes DNA Damage and Apoptosis in Murine Osteoblast-like Cells.
Journal Int J Mol Sci
Abstract Intracellular nutrient metabolism, particularly the metabolism of essential amino acids (EAAs), is crucial for cellular functions, including energy production and redox homeostasis. An EAA deficiency can lead to cellular dysfunction and oxidative stress. This study explores the mechanisms underlying cellular responses to EAA starvation, focusing on ROS-induced DNA damage and apoptosis. MC3T3-E1 cells were subjected to EAA starvation, and various assays were conducted to assess cell proliferation, survival, DNA damage, and apoptosis. The antioxidant N-acetylcysteine (NAC) was employed to block ROS formation and mitigate cellular damage. Gene expression and Western blot analyses were performed to elucidate molecular pathways. EAA starvation-induced ROS generation, DNA damage, and apoptosis in MC3T3-E1 cells. NAC administration effectively reduced DNA damage and apoptosis, highlighting the pivotal role of ROS in mediating these cellular responses during EAA deficiency. This study demonstrates that EAA starvation triggers ROS-mediated DNA damage and apoptosis, offering insights into the intricate interplay between nutrient deficiency, oxidative stress, and programmed cell death. NAC emerges as a potential therapeutic intervention to counteract these adverse effects.
Volume 24(20)
Published 2023-10-18
DOI 10.3390/ijms242015314
PII ijms242015314
PMID 37894999
PMC PMC10607495
MeSH Acetylcysteine / metabolism Acetylcysteine / pharmacology Amino Acids, Essential / metabolism Animals Apoptosis* DNA Damage Mice Osteoblasts / metabolism Oxidative Stress* Reactive Oxygen Species / metabolism
Human and Animal Cells MC3T3-E1(RCB1126)