RRC ID 78776
Author Zhang D, Shimokawa T, Guo Q, Dan S, Miki Y, Sunada S.
Title Discovery of novel DNA-damaging agents through phenotypic screening for DNA double-strand break.
Journal Cancer Sci
Abstract DNA double-strand breaks (DSBs) seriously damage DNA and promote genomic instability that can lead to cell death. They are the source of conditions such as carcinogenesis and aging, but also have important applications in cancer therapy. Therefore, rapid detection and quantification of DSBs in cells are necessary for identifying carcinogenic and anticancer factors. In this study, we detected DSBs using a flow cytometry-based high-throughput method to analyze γH2AX intensity. We screened a chemical library containing 9600 compounds and detected multiple DNA-damaging compounds, although we could not identify mechanisms of action through this procedure. Thus, we also profiled a representative compound with the highest DSB potential, DNA-damaging agent-1 (DDA-1), using a bioinformatics-based method we termed "molecular profiling." Prediction and verification analysis revealed DDA-1 as a potential inhibitor of topoisomerase IIα, different from known inhibitors such as etoposide and doxorubicin. Additional investigation of DDA-1 analogs and xenograft models suggested that DDA-1 is a potential anticancer drug. In conclusion, our findings established that combining high-throughput DSB detection and molecular profiling to undertake phenotypic analysis is a viable method for efficient identification of novel DNA-damaging compounds for clinical applications.
Volume 114(3)
Pages 1108-1117
Published 2023-3-1
DOI 10.1111/cas.15659
PMID 36385507
PMC PMC9986057
MeSH Antineoplastic Agents* DNA DNA Breaks, Double-Stranded* DNA Damage DNA Repair Etoposide / pharmacology Histones / metabolism Humans
Human and Animal Cells A549(RCB0098)