| RRC ID |
78885
|
| 著者 |
Taniguchi K, Nishiura H, Ota Y, Yamamoto T.
|
| タイトル |
Roles of the ribosomal protein S19 dimer and chemically induced apoptotic cells as a tumor vaccine in syngeneic mouse transplantation models.
|
| ジャーナル |
J Immunother
|
| Abstract |
We examined the roles of a chemotherapeutic reagent in both inducing apoptosis and conferring acquired tumor immunity using mouse syngeneic tumor transplantation models. A chemotherapeutic reagent, cis-diamminedichloroplatinum (II), effectively induced apoptosis in Colon-26 cells originating from a BALB/c mouse. Three intradermal inoculations of cis-diamminedichloroplatinum (II)-treated Colon-26 cells conferred tumor immunity against viable Colon-26 cells in BALB/c mice but not in athymic BALB/c mice. The acquired immunity was Colon-26 cell specific and was adoptively transferable with splenic cells. We next examined the involvement of the cross-linked ribosomal protein S19 dimer (RP S19), which is released during apoptosis, in the acquisition of tumor immunity. A Colon-26 transformant that produces a Gln137Asn-mutant RP S19 prevents the formation of a functional RP S19 dimer. Acquired tumor immunity was significantly reduced when the transformant was used in combination with anti-RP S19 antibodies as the vaccination source. These data suggest the importance of the RP S19 dimer in chemotherapeutic agent-induced acquired immunity.
|
| 巻・号 |
34(1)
|
| ページ |
16-27
|
| 公開日 |
2011-1-1
|
| DOI |
10.1097/CJI.0b013e3181fb03ed
|
| PII |
00002371-201101000-00002
|
| PMID |
21150710
|
| MeSH |
Adoptive Transfer
Animals
Apoptosis*
Blotting, Western
Cancer Vaccines / immunology*
Cell Line, Tumor
Cisplatin / pharmacology
Immunotherapy, Adoptive
In Situ Nick-End Labeling
Injections, Intradermal
Mice
Mice, Inbred BALB C
Mice, Nude
Mutation
Neoplasm Transplantation
Neoplasms, Experimental
Protein Multimerization
Ribosomal Proteins / immunology*
Signal Transduction
Transplantation, Isogeneic
|
| リソース情報 |
| ヒト・動物細胞 |
Meth-A(RCB0464) |
