RRC ID 78885
Author Taniguchi K, Nishiura H, Ota Y, Yamamoto T.
Title Roles of the ribosomal protein S19 dimer and chemically induced apoptotic cells as a tumor vaccine in syngeneic mouse transplantation models.
Journal J Immunother
Abstract We examined the roles of a chemotherapeutic reagent in both inducing apoptosis and conferring acquired tumor immunity using mouse syngeneic tumor transplantation models. A chemotherapeutic reagent, cis-diamminedichloroplatinum (II), effectively induced apoptosis in Colon-26 cells originating from a BALB/c mouse. Three intradermal inoculations of cis-diamminedichloroplatinum (II)-treated Colon-26 cells conferred tumor immunity against viable Colon-26 cells in BALB/c mice but not in athymic BALB/c mice. The acquired immunity was Colon-26 cell specific and was adoptively transferable with splenic cells. We next examined the involvement of the cross-linked ribosomal protein S19 dimer (RP S19), which is released during apoptosis, in the acquisition of tumor immunity. A Colon-26 transformant that produces a Gln137Asn-mutant RP S19 prevents the formation of a functional RP S19 dimer. Acquired tumor immunity was significantly reduced when the transformant was used in combination with anti-RP S19 antibodies as the vaccination source. These data suggest the importance of the RP S19 dimer in chemotherapeutic agent-induced acquired immunity.
Volume 34(1)
Pages 16-27
Published 2011-1-1
DOI 10.1097/CJI.0b013e3181fb03ed
PII 00002371-201101000-00002
PMID 21150710
MeSH Adoptive Transfer Animals Apoptosis* Blotting, Western Cancer Vaccines / immunology* Cell Line, Tumor Cisplatin / pharmacology Immunotherapy, Adoptive In Situ Nick-End Labeling Injections, Intradermal Mice Mice, Inbred BALB C Mice, Nude Mutation Neoplasm Transplantation Neoplasms, Experimental Protein Multimerization Ribosomal Proteins / immunology* Signal Transduction Transplantation, Isogeneic
IF 4.11
Human and Animal Cells Meth-A(RCB0464)