Reference - Detail
| RRC ID | 79477 |
|---|---|
| Author | Shinohara N, Ogiso Y, Tanaka M, Sazawa A, Harabayashi T, Koyanagi T. |
| Title | The significance of Ras guanine nucleotide exchange factor, son of sevenless protein, in renal cell carcinoma cell lines. |
| Journal | J Urol |
| Abstract |
PURPOSE:The aim of the present study is to clarify the significance of the Ras guanine-nucleotide exchange reaction in the proliferation of human renal cell carcinoma cell lines. MATERIALS AND METHODS:We examined the expression of human son of sevenless-1 (hSos-1) protein and the epidermal growth factor (EGF) receptor in human renal cell carcinoma cell lines by Western blot analysis. Additionally, a dominant negative H-ras mutant, N116Y, which is known to inhibit the Ras guanine-nucleotide exchange reaction, was transfected into these cell lines by lipofection. RESULTS:Human renal cell carcinoma cell lines expressed much higher amounts of the EGF receptor and hSos-1 protein than normal kidney tissue. Moreover, the N116Y ras mutant could strongly suppress cellular proliferation in these cell lines. CONCLUSIONS:Augmentation of the Ras guanine-nucleotide exchange reaction might be essential to the proliferation of human renal cell carcinoma cells. |
| Volume | 158(3 Pt 1) |
| Pages | 908-11 |
| Published | 1997-9-1 |
| DOI | 10.1097/00005392-199709000-00070 |
| PII | S0022-5347(01)64362-3 |
| PMID | 9258117 |
| MeSH | Carcinoma, Renal Cell / genetics* Carcinoma, Renal Cell / pathology Cell Division ErbB Receptors / genetics* Eukaryotic Initiation Factor-2 / genetics* Gene Expression Regulation, Neoplastic Genes, ras / genetics Guanine Nucleotide Exchange Factors Humans Kidney Neoplasms / genetics* Kidney Neoplasms / pathology Mutation Proteins / genetics* Tumor Cells, Cultured ras Guanine Nucleotide Exchange Factors |
| IF | 5.925 |
| Resource | |
| Human and Animal Cells | OS-RC-2(RCB0735) |