RRC ID 81009
Author Dong Z, Hu B, Wang S, Wang M, Sun S, Liu X, Li D, Wu D.
Title LncRNA MAGI2-AS3 promotes fracture healing through downregulation of miR-223-3p.
Journal J Orthop Surg Res
Abstract BACKGROUND:Long non-coding RNAs (LncRNAs) are recognized as a pivotal element in the processes of fracture healing and the osteogenic differentiation of stem cells. This study investigated the molecular mechanism and regulatory significance of lncRNA MAGI2-AS3 (MAGI2-AS3) in fracture healing.
METHODS:Serum levels of MAGI2-AS3 in patients with normal and delayed fracture healing were verified by RT-qPCR assays. The predictive efficacy of MAGI2-AS3 for delayed fracture healing was analyzed by ROC curve. Osteogenic markers were quantified by RT-qPCR assays. MC3T3-E1 cell viability was detected using CCK-8 assay, and flow cytometry was utilized to measure cell apoptosis. The dual-luciferase reporter gene assay was used to determine the targeted binding between MAGI2-AS3 and miR-223-3p.
RESULTS:Serum MAGI2-AS3 expression was decreased in patients with delayed fracture healing compared with patients with normal healing. Elevated MAGI2-AS3 resulted in an upregulation of the proliferative capacity of MC3T3-E1 cells and a decrease in mortality, along with increased levels of both osteogenic markers. However, after transfection silencing MAGI2-AS3, the trend was reversed. Additionally, miR-223-3p was the downstream target of MAGI2-AS3 and was controlled by MAGI2-AS3. miR-223-3p mimic reversed the promoting effects of MAGI2-AS3 overexpression on osteogenic marker levels and cell growth, and induced cell apoptosis.
CONCLUSION:The upregulation of MAGI2-AS3 may expedite the healing of fracture patients by targeting miR-223-3p, offering a novel biomarker for diagnosing patients with delayed healing.
Volume 19(1)
Pages 370
Published 2024-6-22
DOI 10.1186/s13018-024-04850-5
PII 10.1186/s13018-024-04850-5
PMID 38907263
PMC PMC11193218
MeSH Adaptor Proteins, Signal Transducing / genetics Adult Animals Apoptosis / genetics Cell Differentiation / genetics Cell Proliferation / genetics Down-Regulation* Female Fracture Healing* / genetics Fracture Healing* / physiology Humans Male Mice MicroRNAs* / genetics Osteogenesis / genetics RNA, Long Noncoding* / genetics
IF 1.777
Resource
Human and Animal Cells MC3T3-E1(RCB1126)