RRC ID 81890
Author Orimoto A, Kashiwagi S, Funakoshi A, Shimizu T, Ishii T, Kiyono T, Fukuda T.
Title Transcriptome-wide profiling for melanocytes derived from newborn and adult human epidermis with enhanced proliferation.
Journal Cell Biol Int
Abstract The senescence-associated protein p16INK4A acts as a limiter element in cell-cycle progression. The loss of p16INK4A function is causally related to cellular immortalization. The increase in p16INK4A levels with advancing age was demonstrated in melanocytes. However, the characteristic difference between young and senescent melanocytes affecting immortalization of melanocytes remains unclear. In this study, we generated 10 different cell lines in total from newborn (NB) and adult (AD) primary normal human epidermal melanocytes (NHEM) using four different methods, transduction of CDK4R24C and cyclin D1 (K4D), K4D with TERT (K4DT), SV40 T-antigen (SV40T), and HPV16 E6 and E7 (E6/E7) and performed whole transcriptome sequencing analysis (RNA-Seq) to elucidate the differences of genome-wide expression profiles among cell lines. The analysis data revealed distinct differences in expression pattern between cell lines from NB and AD although no distinct biological differences were detected in analyses such as comparison of cell morphology, evaluation of cell proliferation, and cell cycle profiles. This study may provide useful in vitro models to benefit the understanding of skin-related diseases.
Volume 48(10)
Pages 1573-1587
Published 2024-10-1
DOI 10.1002/cbin.12214
PMID 38961533
MeSH Adult Cell Cycle / genetics Cell Line Cell Proliferation* Cells, Cultured Cellular Senescence / genetics Cyclin-Dependent Kinase 4 / genetics Cyclin-Dependent Kinase 4 / metabolism Epidermal Cells / cytology Epidermal Cells / metabolism Epidermis* / metabolism Gene Expression Profiling* Humans Infant, Newborn Melanocytes* / cytology Melanocytes* / metabolism Transcriptome
Resource
DNA material pCMV-VSV-G-RSV-Rev (RDB04393)