Reference - Detail
| RRC ID | 81972 |
|---|---|
| Author | Gao P, Ma X, Yuan M, Yi Y, Liu G, Wen M, Jiang W, Ji R, Zhu L, Tang Z, Yu Q, Xu J, Yang R, Xia S, Yang M, Pan J, Yuan H, An H. |
| Title | E3 ligase Nedd4l promotes antiviral innate immunity by catalyzing K29-linked cysteine ubiquitination of TRAF3. |
| Journal | Nat Commun |
| Abstract |
Ubiquitination is one of the most prevalent protein posttranslational modifications. Here, we show that E3 ligase Nedd4l positively regulates antiviral immunity by catalyzing K29-linked cysteine ubiquitination of TRAF3. Deficiency of Nedd4l significantly impairs type I interferon and proinflammatory cytokine production induced by virus infection both in vitro and in vivo. Nedd4l deficiency inhibits virus-induced ubiquitination of TRAF3, the binding between TRAF3 and TBK1, and subsequent phosphorylation of TBK1 and IRF3. Nedd4l directly interacts with TRAF3 and catalyzes K29-linked ubiquitination of Cys56 and Cys124, two cysteines that constitute zinc fingers, resulting in enhanced association between TRAF3 and E3 ligases, cIAP1/2 and HECTD3, and also increased K48/K63-linked ubiquitination of TRAF3. Mutation of Cys56 and Cys124 diminishes Nedd4l-catalyzed K29-linked ubiquitination, but enhances association between TRAF3 and the E3 ligases, supporting Nedd4l promotes type I interferon production in response to virus by catalyzing ubiquitination of the cysteines in TRAF3. |
| Volume | 12(1) |
| Pages | 1194 |
| Published | 2021-2-19 |
| DOI | 10.1038/s41467-021-21456-1 |
| PII | 10.1038/s41467-021-21456-1 |
| PMID | 33608556 |
| PMC | PMC7895832 |
| MeSH | Animals Antiviral Agents / pharmacology* Baculoviral IAP Repeat-Containing 3 Protein / metabolism Catalysis Cysteine / metabolism Female HEK293 Cells Humans Immunity, Innate / drug effects* Inhibitor of Apoptosis Proteins / metabolism Interferon Regulatory Factor-3 / metabolism Interferon Type I / metabolism Mice Mice, Inbred C57BL Mice, Knockout Nedd4 Ubiquitin Protein Ligases / deficiency Nedd4 Ubiquitin Protein Ligases / genetics* Nedd4 Ubiquitin Protein Ligases / metabolism* Protein Processing, Post-Translational Protein Serine-Threonine Kinases TNF Receptor-Associated Factor 3 / genetics TNF Receptor-Associated Factor 3 / metabolism* Ubiquitin-Protein Ligases / genetics Ubiquitin-Protein Ligases / metabolism* Ubiquitination* |
| IF | 12.121 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 293T(RCB2202) |