RRC ID 82040
Author Shimomura H, Okada R, Tanaka T, Hozaka Y, Wada M, Moriya S, Idichi T, Kita Y, Kurahara H, Ohtsuka T, Seki N.
Title Role of miR-30a-3p Regulation of Oncogenic Targets in Pancreatic Ductal Adenocarcinoma Pathogenesis.
Journal Int J Mol Sci
Abstract Our recent studies have implicated some passenger strands of miRNAs in the molecular pathogenesis of human cancers. Analysis of the microRNA (miRNA) expression signature in pancreatic ductal adenocarcinoma (PDAC) has shown that levels of miR-30a-3p, the passenger strand derived from pre-mir-30a, are significantly downregulated in PDAC tissues. This study aimed to identify the oncogenes closely involved in PDAC molecular pathogenesis under the regulation of miR-30a-3p. Ectopic expression assays showed that miR-30a-3p expression inhibited the aggressiveness of the PDAC cells, suggesting that miR-30a-3p acts as a tumor-suppressive miRNA in PDAC cells. We further identified 102 putative targets of miR-30a-3p regulation in PDAC cells by combining in silico analysis with gene expression data. Of these, ten genes (EPS8, HMGA2, ENDOD1, SLC39A10, TGM2, MGLL, SERPINE1, ITGA2, DTL, and UACA) were independent prognostic factors in multivariate analysis of survival of patients with PDAC (p < 0.01). We also investigated the oncogenic function of the integrin ITGA2 in PDAC cell lines. The integrin family comprises cell adhesion molecules expressed as heterodimeric, transmembrane proteins on the surface of various cells. Overexpression of ITGA2/ITGB1 (an ITGA2 binding partner) was detected in the PDAC clinical specimens. The knockdown of ITGA2 expression attenuated the malignant phenotypes of the PDAC cells. Together, results from these microRNA-based approaches can accelerate our understanding of PDAC molecular pathogenesis.
Volume 21(18)
Published 2020-9-4
DOI 10.3390/ijms21186459
PII ijms21186459
PMID 32899691
PMC PMC7555373
MeSH Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism Adult Carcinogenesis / genetics Carcinoma, Pancreatic Ductal / genetics* Carcinoma, Pancreatic Ductal / pathology* Cell Line, Tumor Cell Movement / genetics Cell Proliferation / genetics Female Gene Expression / genetics Gene Expression Regulation, Neoplastic / genetics Humans Male MicroRNAs / genetics* MicroRNAs / physiology Middle Aged Oncogenes Pancreatic Neoplasms / genetics Pancreatic Neoplasms / pathology Signal Transduction / genetics Transcriptome / genetics
Resource
Human and Animal Cells PANC-1(RCB2095)