| RRC ID |
82048
|
| 著者 |
Nakai-Futatsugi Y, Jin J, Ogawa T, Sakai N, Maeda A, Hironaka KI, Fukuda M, Danno H, Tanaka Y, Hori S, Shiroguchi K, Takahashi M.
|
| タイトル |
Pigmentation level of human iPSC-derived RPE does not indicate a specific gene expression profile.
|
| ジャーナル |
Elife
|
| Abstract |
Retinal pigment epithelium (RPE) cells show heterogeneous levels of pigmentation when cultured in vitro. To know whether their color in appearance is correlated with the function of the RPE, we analyzed the color intensities of human-induced pluripotent stem cell-derived RPE cells (iPSC-RPE) together with the gene expression profile at the single-cell level. For this purpose, we utilized our recent invention, Automated Live imaging and cell Picking System (ALPS), which enabled photographing each cell before RNA-sequencing analysis to profile the gene expression of each cell. While our iPSC-RPE were categorized into four clusters by gene expression, the color intensity of iPSC-RPE did not project any specific gene expression profiles. We reasoned this by less correlation between the actual color and the gene expressions that directly define the level of pigmentation, from which we hypothesized the color of RPE cells may be a temporal condition not strongly indicating the functional characteristics of the RPE.
|
| 巻・号 |
12
|
| 公開日 |
2024-5-9
|
| DOI |
10.7554/eLife.92510
|
| PII |
92510
|
| PMID |
38722314
|
| PMC |
PMC11081631
|
| MeSH |
Cell Differentiation / genetics
Cells, Cultured
Gene Expression Profiling
Humans
Induced Pluripotent Stem Cells* / metabolism
Pigmentation* / genetics
Retinal Pigment Epithelium* / cytology
Retinal Pigment Epithelium* / metabolism
Retinal Pigment Epithelium* / physiology
Transcriptome*
|
| IF |
7.08
|
| リソース情報 |
| ヒト・動物細胞 |
201B7-Ff(HPS4290)
253G1(HPS0002) |
