論文 - 詳細
| RRC ID | 83735 |
|---|---|
| 著者 | Chen P, Pan KC, Park EH, Luo Y, Lee YCG, Aravin AA. |
| タイトル | Escalation of genome defense capacity enables control of an expanding meiotic driver. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
From RNA interference to chromatin silencing, diverse genome defense pathways silence selfish genetic elements to safeguard genome integrity. Despite their diversity, different defense pathways share a modular organization, where numerous specificity factors identify diverse targets and common effectors silence them. In the PIWI-interacting RNA (piRNA) pathway, target RNAs are first identified by complementary base pairing with piRNAs and then silenced by PIWI-clade nucleases. Such a binary architecture allows the defense systems to be readily adaptable, where new targets can be captured via innovation of specificity factors. Thus, our current understanding of genome defense against lineage-specific selfish genes has been largely limited to specificity factor innovations, while it remains poorly understood whether other types of innovations are required. Here, we describe a new type of innovation, which escalates the genome defense capacity to control a recently expanded selfish gene in Drosophila melanogaster. Through a targeted RNAi screen for repressors of Stellate-a recently evolved meiotic driver-we identified a defense factor, Trailblazer. Trailblazer is a transcription factor that promotes the expression of two PIWI-clade nucleases, Aub and AGO3, to match Stellate in abundance. Recent innovation in the DNA-binding domain of Trailblazer enabled it to elevate Aub and AGO3 expression, thereby escalating the silencing capacity of piRNA pathway to tame expanded Stellate and safeguard fertility. As copy-number expansion is a recurrent feature of diverse selfish genes across the tree of life, we envision that augmenting the defense capacity to quantitatively match selfish genes is a repeatedly employed defense strategy in evolution. |
| 巻・号 | 122(2) |
| ページ | e2418541122 |
| 公開日 | 2025-1-14 |
| DOI | 10.1073/pnas.2418541122 |
| PMID | 39772737 |
| PMC | PMC11745323 |
| MeSH | Animals DNA / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster* / cytology Drosophila melanogaster* / genetics Genome, Insect* Meiosis* Protein Binding RNA Interference Repetitive Sequences, Nucleic Acid Transcription Factors / metabolism Up-Regulation |
| IF | 9.412 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#104055 |