論文 - 詳細
| RRC ID | 84020 |
|---|---|
| 著者 | Lyu H, Chamberlin HM. |
| タイトル | Functional distinction in oncogenic Ras variant activity in Caenorhabditis elegans. |
| ジャーナル | Dis Model Mech |
| Abstract |
Ras genes are important oncogenes that are frequently mutated in cancer. Human oncogenic variants exhibit functional distinctions in terms of their representation in different cancer types, impact on cellular targets and sensitivity to pharmacological treatments. However, how these distinct variants influence and respond to the cellular networks in which they are embedded is poorly understood. To identify novel participants in the complex interplay between Ras genotype and cell interaction networks in vivo, we have developed and tested an experimental framework using a simple vulva-development assay in the nematode C. elegans. Using this system, we evaluated a set of Ras oncogenic substitution changes at G12, G13 and Q61. We found that these variants fall into distinct groups based on phenotypic differences, sensitivity to gene dosage and inhibition of the downstream kinase MEK and their response to genetic modulators that influence Ras activity in a non-autonomous manner. Together, our results demonstrated that oncogenic C. elegans Ras variants exhibit clear distinctions in how they interface with the vulva-development network and showed that extracellular modulators yield variant-restricted effects in vivo. |
| 巻・号 | 17(8) |
| 公開日 | 2024-8-1 |
| DOI | 10.1242/dmm.050577 |
| PII | 359246 |
| PMID | 38946472 |
| PMC | PMC11340813 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Female Humans Mutation / genetics Oncogenes / genetics Phenotype Vulva* / metabolism Vulva* / pathology ras Proteins* / genetics ras Proteins* / metabolism |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm810 |