RRC ID 861
Author Hishida T, Han YW, Shibata T, Kubota Y, Ishino Y, Iwasaki H, Shinagawa H.
Title Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks.
Journal Genes Dev.
Abstract The RecQ protein family is a highly conserved group of DNA helicases that play roles in maintaining genomic stability. In this study, we present biochemical and genetic evidence that Escherichia coli RecQ processes stalled replication forks and participates in SOS signaling. Cells that carry dnaE486, a mutation in the DNA polymerase III alpha-catalytic subunit, induce an RecA-dependent SOS response and become highly filamented at the semirestrictive temperature (38 degrees C). An recQ mutation suppresses the induction of SOS response and the filamentation in the dnaE486 mutant at 38 degrees C, causing appearance of a high proportion of anucleate cells. In vitro, RecQ binds and unwinds forked DNA substrates with a gap on the leading strand more efficiently than those with a gap on the lagging strand or Holliday junction DNA. RecQ unwinds the template duplex ahead of the fork, and then the lagging strand is unwound. Consequently, this process generates a single-stranded DNA (ssDNA) gap on the lagging strand adjacent to a replication fork. These results suggest that RecQ functions to generate an initiating signal that can recruit RecA for SOS induction and recombination at stalled replication forks, which are required for the cell cycle checkpoint and resumption of DNA replication.
Volume 18(15)
Pages 1886-97
Published 2004-8-1
DOI 10.1101/gad.1223804
PII 18/15/1886
PMID 15289460
PMC PMC517408
MeSH Adenosine Triphosphatases / physiology* DNA Helicases / physiology* DNA Polymerase III / genetics DNA Polymerase III / metabolism DNA Replication* DNA, Bacterial / genetics DNA, Bacterial / metabolism DNA, Single-Stranded / genetics DNA, Single-Stranded / metabolism Escherichia coli / enzymology* Escherichia coli / genetics Genome, Bacterial* Mutation / genetics RecQ Helicases Recombinant Proteins / genetics Recombinant Proteins / metabolism Signal Transduction / physiology* Son of Sevenless Proteins / metabolism* Temperature
IF 9.462
Times Cited 80
WOS Category GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY CELL BIOLOGY
Resource
Prokaryotes E. coli ME8680(E486) ME8447(KD1996) ME8785(CAG18436) ME8081(AB1886)