| RRC ID |
861
|
| 著者 |
Hishida T, Han YW, Shibata T, Kubota Y, Ishino Y, Iwasaki H, Shinagawa H.
|
| タイトル |
Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks.
|
| ジャーナル |
Genes Dev
|
| Abstract |
The RecQ protein family is a highly conserved group of DNA helicases that play roles in maintaining genomic stability. In this study, we present biochemical and genetic evidence that Escherichia coli RecQ processes stalled replication forks and participates in SOS signaling. Cells that carry dnaE486, a mutation in the DNA polymerase III alpha-catalytic subunit, induce an RecA-dependent SOS response and become highly filamented at the semirestrictive temperature (38 degrees C). An recQ mutation suppresses the induction of SOS response and the filamentation in the dnaE486 mutant at 38 degrees C, causing appearance of a high proportion of anucleate cells. In vitro, RecQ binds and unwinds forked DNA substrates with a gap on the leading strand more efficiently than those with a gap on the lagging strand or Holliday junction DNA. RecQ unwinds the template duplex ahead of the fork, and then the lagging strand is unwound. Consequently, this process generates a single-stranded DNA (ssDNA) gap on the lagging strand adjacent to a replication fork. These results suggest that RecQ functions to generate an initiating signal that can recruit RecA for SOS induction and recombination at stalled replication forks, which are required for the cell cycle checkpoint and resumption of DNA replication.
|
| 巻・号 |
18(15)
|
| ページ |
1886-97
|
| 公開日 |
2004-8-1
|
| DOI |
10.1101/gad.1223804
|
| PII |
18/15/1886
|
| PMID |
15289460
|
| PMC |
PMC517408
|
| MeSH |
Adenosine Triphosphatases / physiology*
DNA Helicases / physiology*
DNA Polymerase III / genetics
DNA Polymerase III / metabolism
DNA Replication*
DNA, Bacterial / genetics
DNA, Bacterial / metabolism
DNA, Single-Stranded / genetics
DNA, Single-Stranded / metabolism
Escherichia coli / enzymology*
Escherichia coli / genetics
Genome, Bacterial*
Mutation / genetics
RecQ Helicases
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Signal Transduction / physiology*
Son of Sevenless Proteins / metabolism*
Temperature
|
| IF |
9.527
|
| 引用数 |
95
|
|
WOS 分野
|
GENETICS & HEREDITY
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
ME8680(E486)
ME8447(KD1996)
ME8785(CAG18436)
ME8081(AB1886) |
