論文 - 詳細
| RRC ID | 86111 |
|---|---|
| 著者 | Aida R, Okano K, Nakata K, Hagiwara H. |
| タイトル | Apigenin-induced Apoptosis in Lung Adenocarcinoma A549 Cells: Involvement in IFNA2, TNF, and SPON2 With Different Time Points. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Apigenin, found in a variety of vegetables and fruits, exhibits anti-oxidant, anti-inflammatory and anticancer effects. Recently, we reported the possibility that apigenin induces apoptosis in human lung adenocarcinoma A549 cells through the miR-34a-5p/SNAI1/caspase-3/-7 pathway. Understanding how apigenin triggers apoptosis in cancer cells will help lay the groundwork for developing effective cancer treatments. MATERIALS AND METHODS:The lung adenocarcinoma A549 Cell line was used. To determine whether caspase-8 or caspase-9 is activated, we performed a caspase activity assay. Real-time qRT-PCR was performed to identify mRNAs that may stimulate the upstream pathways, including tumor necrosis factor-a (TNF-a), spondin-2 (SPON2), and interferon-a2 (IFNA2), which are known to be involved in apoptosis in various cancer cell lines. RESULTS:In apigenin-treated cells, early-stage apoptosis was observed at 24 h, with increased activity of caspase-8 at 18 h and again at 24 h, and caspase-9 at 24 h and further at 48 h. However, mRNA levels of caspase-8 and caspase-9 significantly decreased after 24 h. Real-time RT-qPCR analysis revealed increased mRNA levels of TNF-a, spondin-2, and interferon-a2 after 24 h of apigenin treatment in A549 cells, whereas treatment for 48 h led to decreased expression of SPON2 and IFNA2. CONCLUSION:Apigenin promotes apoptosis in A549 cells by modulating various signaling pathways at different time points. |
| 巻・号 | 45(7) |
| ページ | 2781-2790 |
| 公開日 | 2025-7-1 |
| DOI | 10.21873/anticanres.17647 |
| PII | 45/7/2781 |
| PMID | 40578952 |
| MeSH | A549 Cells Adenocarcinoma of Lung* / drug therapy Adenocarcinoma of Lung* / genetics Adenocarcinoma of Lung* / metabolism Adenocarcinoma of Lung* / pathology Apigenin* / pharmacology Apoptosis* / drug effects Caspase 8 / genetics Caspase 8 / metabolism Caspase 9 / metabolism Extracellular Matrix Proteins* / genetics Extracellular Matrix Proteins* / metabolism Gene Expression Regulation, Neoplastic / drug effects Humans Lung Neoplasms* / drug therapy Lung Neoplasms* / genetics Lung Neoplasms* / metabolism Lung Neoplasms* / pathology Signal Transduction / drug effects Tumor Necrosis Factor-alpha* / genetics Tumor Necrosis Factor-alpha* / metabolism |
| IF | 1.994 |
| リソース情報 | |
| ヒト・動物細胞 | A549 |