論文 - 詳細
| RRC ID | 87265 |
|---|---|
| 著者 | Horii T, Yamamoto M, Morita S, Kimura M, Nagao Y, Hatada I. |
| タイトル | p53 suppresses tetraploid development in mice. |
| ジャーナル | Sci Rep |
| Abstract |
Mammalian tetraploid embryos die in early development because of defects in the epiblast. Experiments with diploid/tetraploid chimeric mice, obtained via the aggregation of embryonic stem cells, clarified that while tetraploid cells are excluded from epiblast derivatives, diploid embryos with tetraploid extraembryonic tissues can develop to term. Today, this method, known as tetraploid complementation, is usually used for rescuing extraembryonic defects or for obtaining completely embryonic stem (ES) cell-derived pups. However, it is still unknown why defects occur in the epiblast during mammalian development. Here, we demonstrated that downregulation of p53, a tumour suppressor protein, rescued tetraploid development in the mammalian epiblast. Tetraploidy in differentiating epiblast cells triggered p53-dependent cell-cycle arrest and apoptosis, suggesting the activation of a tetraploidy checkpoint during early development. Finally, we found that p53 downregulation rescued tetraploid embryos later in gestation. |
| 巻・号 | 5 |
| ページ | 8907 |
| 公開日 | 2015-3-10 |
| DOI | 10.1038/srep08907 |
| PII | srep08907 |
| PMID | 25752699 |
| PMC | PMC4354145 |
| MeSH | Animals Apoptosis / genetics Blastocyst / metabolism Cell Cycle Checkpoints / genetics Chimera Embryo, Mammalian Embryonic Development / genetics* Gene Expression Regulation, Developmental Mice Tetraploidy* Tumor Suppressor Protein p53 / biosynthesis Tumor Suppressor Protein p53 / genetics* |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC01361 RBRC00213 |