RRC ID 880
Author Nakase Y, Nakamura T, Okazaki K, Hirata A, Shimoda C.
Title The Sec14 family glycerophospholipid-transfer protein is required for structural integrity of the spindle pole body during meiosis in fission yeast.
Journal Genes Cells
Abstract The fission yeast spo20+ gene encodes a glycerophospholipid-transfer protein. spo20 mutants are unable to assemble the forespore membrane properly. Here we studied the structural integrity of the spindle pole body (SPB) in spo20-H6 mutants during meiosis. Meiotic cells expressing a GFP-tagged SPB marker protein, Spo15-GFP, showed an excess number of SPBs, some of which were not localized to the spindle poles and were termed 'pseudo-SPBs'. Formation of spindles for meiosis I was significantly delayed in spo20-H6 cells, although the morphology of spindles and segregation of the sister chromatids seemed normal. The SPB of spo20-H6 contained meiosis-specific outer plaques, though outermost layers were less evident. Time-lapse studies of spo20-H6 cells showed that the pseudo-SPBs originated from normal SPBs at the spindle poles during meiosis I. Among the SPB components tested, Spo15, Spo13, Sad1 and Cut12 were localized to the pseudo-SPBs, but Sid4 was not always present. Alp4, a component of the gamma-tubulin complex, was also present in about 40% of the pseudo-SPBs. The forespore membranes initiated from both the SPBs and the pseudo-SPBs. We conclude that Spo20 plays a role in maintaining the structural integrity of the meiotic SPB, besides supplying membrane vesicles for forespore membrane assembly.
Volume 9(12)
Pages 1275-86
Published 2004-12
DOI 10.1111/j.1365-2443.2004.00806.x
PII GTC806
PMID 15569158
MeSH Alleles Base Sequence Genetic Vectors Glycerophospholipids / metabolism Meiosis* Molecular Sequence Data Mutation Phospholipid Transfer Proteins / genetics* Phospholipid Transfer Proteins / physiology* Plasmids Schizosaccharomyces / cytology Schizosaccharomyces / genetics Schizosaccharomyces / ultrastructure* Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / physiology* Spindle Apparatus / chemistry Spindle Apparatus / ultrastructure*
IF 2.048
Times Cited 11
WOS Category GENETICS & HEREDITY CELL BIOLOGY
Resource
Yeast YN12 TN8 FY12252(YN26S) FY12262(YN34) FY12296(YN68) FY12317(YN89) FY12366(YN138) FY12375(YN147) FY12464(YN286) FY12519(YN341) etc.