論文 - 詳細
| RRC ID | 88073 |
|---|---|
| 著者 | Dai W, Zhai Y, Yang F, Chen W, Liu C, Tian Y, Huang F, Cai M, Zheng L, Cheng W, Chen W, Zhang J. |
| タイトル | Different concentrations of 2-Undecanone triggers repellent and nematicidal responses in Caenorhabditis elegans. |
| ジャーナル | Sci Rep |
| Abstract |
The compound 2-undecanone is widely distributed in the natural environment and exhibits a dual-action mechanism against nematodes. It demonstrates repellency and contact toxicity against both Caenorhabditis elegans and Meloidogyne incognita. However, research on the dual-function mechanism of 2-undecanone remains relatively limited. In this study, using chemotaxis experiments, we found that 2-undecanone (at concentrations of 1-5 mg/mL) signal is detected through AWB olfactory sensory neurons in nematode, and then transduced through the cGMP pathway to induce repellent behavior. Moreover, we observed that 2-undecanone (at concentrations of 0.06-0.08 mg/mL) induces intracellular calcium accumulation and causes lysosomal membrane rupture. We further identified Hsp70 A and V-ATPase A as the targets of 2-undecanone responsible for its contact killing effect. Furthermore, 2-undecanone was found to alter sphingomyelin metabolism in both wild-type C. elegans and hsp-1 mutants. This alteration led to decreased acid sphingomyelinase activity, reduced ceramide levels, and increased sphingomyelin levels. These results indicated that 2-undecanone has dual functions and two target receptors Hsp70 A and V-ATPase A against nematodes, and one AWB olfactory neuron repelled nematodes. The dual-action mode of 2-undecanone can decrease the nematodes' tolerance to the compound appropriately, extending its efficacy duration. Understanding the dual function mechanism of action of 2-undecanone will aid in devising innovative approaches for managing nematode control. |
| 巻・号 | 15(1) |
| ページ | 14186 |
| 公開日 | 2025-4-23 |
| DOI | 10.1038/s41598-025-95332-z |
| PII | 10.1038/s41598-025-95332-z |
| PMID | 40269070 |
| PMC | PMC12019385 |
| MeSH | Animals Antinematodal Agents* / pharmacology Caenorhabditis elegans* / drug effects Caenorhabditis elegans* / metabolism Caenorhabditis elegans* / physiology Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Calcium / metabolism Chemotaxis / drug effects Cyclic GMP / metabolism HSP70 Heat-Shock Proteins / genetics HSP70 Heat-Shock Proteins / metabolism Ketones* / pharmacology Olfactory Receptor Neurons / drug effects Olfactory Receptor Neurons / metabolism Signal Transduction / drug effects Vacuolar Proton-Translocating ATPases / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm5076 tm4559 |