論文 - 詳細
| RRC ID | 88288 |
|---|---|
| 著者 | Zhao T, Fu Y, Zhu J, Liu Y, Zhang Q, Yi Z, Chen S, Jiao Z, Xu X, Xu J, Duo S, Bai Y, Tang C, Li C, Deng H. |
| タイトル | Single-Cell RNA-Seq Reveals Dynamic Early Embryonic-like Programs during Chemical Reprogramming. |
| ジャーナル | Cell Stem Cell |
| Abstract |
Chemical reprogramming provides a powerful platform for exploring the molecular dynamics that lead to pluripotency. Although previous studies have uncovered an intermediate extraembryonic endoderm (XEN)-like state during this process, the molecular underpinnings of pluripotency acquisition remain largely undefined. Here, we profile 36,199 single-cell transcriptomes at multiple time points throughout a highly efficient chemical reprogramming system using RNA-sequencing and reconstruct their progression trajectories. Through identifying sequential molecular events, we reveal that the dynamic early embryonic-like programs are key aspects of successful reprogramming from XEN-like state to pluripotency, including the concomitant transcriptomic signatures of two-cell (2C) embryonic-like and early pluripotency programs and the epigenetic signature of notable genome-wide DNA demethylation. Moreover, via enhancing the 2C-like program by fine-tuning chemical treatment, the reprogramming process is remarkably accelerated. Collectively, our findings offer a high-resolution dissection of cell fate dynamics during chemical reprogramming and shed light on mechanistic insights into the nature of induced pluripotency. |
| 巻・号 | 23(1) |
| ページ | 31-45.e7 |
| 公開日 | 2018-7-5 |
| DOI | 10.1016/j.stem.2018.05.025 |
| PII | S1934-5909(18)30280-7 |
| PMID | 29937202 |
| MeSH | Animals Cells, Cultured Cellular Reprogramming / drug effects* Embryonic Development / drug effects Embryonic Development / genetics* High-Throughput Screening Assays Mice Mice, Inbred C57BL Mice, Transgenic Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / drug effects* Sequence Analysis, RNA* Single-Cell Analysis* Transcriptome |
| IF | 20.86 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 38 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00771 |