論文 - 詳細
| RRC ID | 88500 |
|---|---|
| 著者 | Gorki AD, Symmank D, Zahalka S, Lakovits K, Hladik A, Langer B, Maurer B, Sexl V, Kain R, Knapp S. |
| タイトル | Murine Ex Vivo Cultured Alveolar Macrophages Provide a Novel Tool to Study Tissue-Resident Macrophage Behavior and Function. |
| ジャーナル | Am J Respir Cell Mol Biol |
| Abstract |
Tissue-resident macrophages are of vital importance as they preserve tissue homeostasis in all mammalian organs. Nevertheless, appropriate cell culture models are still limited. Here, we propose a novel culture model to study and expand murine primary alveolar macrophages (AMs), the tissue-resident macrophages of the lung, in vitro over several months. By providing a combination of granulocyte-macrophage colony-stimulating factor, TGFβ, and the PPARγ activator rosiglitazone, we maintain and expand mouse ex vivo cultured AMs (mexAMs) over several months. MexAMs maintain typical morphologic features and stably express primary AM surface markers throughout in vitro culture. They respond to microbial ligands and exhibit an AM-like transcriptional profile, including the expression of AM-specific transcription factors. Furthermore, when transferred into AM-deficient mice, mexAMs efficiently engraft in the lung and fulfill key macrophage functions, leading to a significantly reduced surfactant load in those mice. Altogether, mexAMs provide a novel, simple, and versatile tool to study AM behavior in homeostasis and disease settings. |
| 巻・号 | 66(1) |
| ページ | 64-75 |
| 公開日 | 2022-1-1 |
| DOI | 10.1165/rcmb.2021-0190OC |
| PMID | 34586974 |
| PMC | PMC8803354 |
| MeSH | Animals Animals, Newborn Cells, Cultured Disease Models, Animal Liver / metabolism Lung / pathology Lung / physiopathology Macrophages, Alveolar / metabolism* Macrophages, Alveolar / pathology Mice Mice, Inbred C57BL Phenotype Pulmonary Alveolar Proteinosis / metabolism Pulmonary Alveolar Proteinosis / pathology Pulmonary Alveolar Proteinosis / physiopathology Transcription, Genetic |
| IF | 5.373 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 17 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC06239 |