| Abstract |
An actinobacterial strain, designated DW29H23T, was isolated from greater duckweed Spirodela polyrhiza. The strain was investigated using a polyphasic taxonomy approach. The comparison of the 16S rRNA gene sequence showed that strain DW29H23T was most closely related to Mumia xiangluensis NEAU-KD1T (99.2%) and Mumia quercus NEAU-365T (98.9%). The average nucleotide identity and digital DNA-DNA hybridization values between the strain and closest type strains were below the threshold for species delineation. The strain was Gram-stain-positive, non-spore-forming and non-motile. Cells were irregular, rod-shaped. The whole-cell hydrolysates contained ll-diaminopimelic acid. The whole-cell sugars were glucose and ribose. The polar lipid profiles contained diphosphatidylglycerol, phosphatidylglycerol and three unidentified phospholipids. The major cellular fatty acids (>10%) were C16:0, 10-methyl-C18:0 and C18:1 ω9c. The major menaquinone was MK-9(H4). The G+C content was 71.7%. Genotypic, phenotypic and chemotaxonomic characterizations of strain DW29H23T demonstrated that the strain represents a novel species of the genus Mumia, for which the name Mumia spirodelae sp. nov. is proposed. The type strain is DW29H23T (=TBRC 18998T=NBRC 116930T).
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