RRC ID 901
Author Okuzaki D, Satake W, Hirata A, Nojima H.
Title Fission yeast meu14+ is required for proper nuclear division and accurate forespore membrane formation during meiosis II.
Journal J. Cell. Sci.
Abstract Using a meiosis-specific subtracted cDNA library of Schizosaccharomyces pombe, we identified meu14+ as a gene whose expression is upregulated during meiosis. Transcription of meu14+ is induced abruptly after the cell enters meiosis. Its transcription is dependent on the meiosis-specific transcription factor Mei4. In meu14Delta cells, the segregation and modification of the SPBs (spindle pole bodies) and microtubule elongation during meiosis II were aberrant. Meiotic meu14Delta cells consequently produced a high frequency of abnormal tetranucleate cells harboring aberrant forespore membranes and failed to produce asci. In wild-type cells harboring the integrated meu14+-gfp fusion gene, Meu14-GFP first appeared inside the nuclear region at prophase II, after which it accumulated beside the two SPBs at metaphase II. Thereafter, it formed two ring-shaped structures that surrounded the nucleus at early anaphase II. At post-anaphase II, it disappeared. Meu14-GFP appears to localize at the border of the forespore membrane that later develops into spore walls at the end of sporulation. This was confirmed by coexpressing Spo3-HA, a component of the forespore membrane, with Meu14-GFP. Taken together, we conclude that meu14+ is crucial in meiosis in that it participates in both the nuclear division during meiosis II and the accurate formation of the forespore membrane.
Volume 116(Pt 13)
Pages 2721-35
Published 2003-7-1
DOI 10.1242/jcs.00496
PII jcs.00496
PMID 12759375
MeSH Amino Acid Sequence / genetics Base Sequence / genetics Cell Compartmentation / genetics Cell Cycle Proteins / genetics Cell Cycle Proteins / isolation & purification Cell Cycle Proteins / metabolism* Cell Nucleus / metabolism* Cell Nucleus / ultrastructure Cells, Cultured DNA, Complementary / analysis DNA, Complementary / genetics Fungal Proteins / genetics Fungal Proteins / metabolism Genes, Regulator / genetics Giant Cells / cytology Giant Cells / metabolism Intracellular Membranes / metabolism* Intracellular Membranes / ultrastructure Meiosis / physiology Microscopy, Electron Microtubules / metabolism Molecular Sequence Data Schizosaccharomyces / cytology Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / isolation & purification* Schizosaccharomyces pombe Proteins / metabolism* Spindle Apparatus / genetics Spindle Apparatus / metabolism Spindle Apparatus / ultrastructure Spores, Fungal / metabolism* Spores, Fungal / ultrastructure
IF 4.401
Times Cited 34
WOS Category CELL BIOLOGY
Resource
Yeast CD16-1 FY12692(JZ670) FY7476(AB4) FY7325(TN187) MKD3 FY7236(SI51) YN68