Reference - Detail
| RRC ID | 901 |
|---|---|
| Author | Okuzaki D, Satake W, Hirata A, Nojima H. |
| Title | Fission yeast meu14+ is required for proper nuclear division and accurate forespore membrane formation during meiosis II. |
| Journal | J Cell Sci |
| Abstract |
Using a meiosis-specific subtracted cDNA library of Schizosaccharomyces pombe, we identified meu14+ as a gene whose expression is upregulated during meiosis. Transcription of meu14+ is induced abruptly after the cell enters meiosis. Its transcription is dependent on the meiosis-specific transcription factor Mei4. In meu14Delta cells, the segregation and modification of the SPBs (spindle pole bodies) and microtubule elongation during meiosis II were aberrant. Meiotic meu14Delta cells consequently produced a high frequency of abnormal tetranucleate cells harboring aberrant forespore membranes and failed to produce asci. In wild-type cells harboring the integrated meu14+-gfp fusion gene, Meu14-GFP first appeared inside the nuclear region at prophase II, after which it accumulated beside the two SPBs at metaphase II. Thereafter, it formed two ring-shaped structures that surrounded the nucleus at early anaphase II. At post-anaphase II, it disappeared. Meu14-GFP appears to localize at the border of the forespore membrane that later develops into spore walls at the end of sporulation. This was confirmed by coexpressing Spo3-HA, a component of the forespore membrane, with Meu14-GFP. Taken together, we conclude that meu14+ is crucial in meiosis in that it participates in both the nuclear division during meiosis II and the accurate formation of the forespore membrane. |
| Volume | 116(Pt 13) |
| Pages | 2721-35 |
| Published | 2003-7-1 |
| DOI | 10.1242/jcs.00496 |
| PII | jcs.00496 |
| PMID | 12759375 |
| MeSH | Amino Acid Sequence / genetics Base Sequence / genetics Cell Compartmentation / genetics Cell Cycle Proteins / genetics Cell Cycle Proteins / isolation & purification Cell Cycle Proteins / metabolism* Cell Nucleus / metabolism* Cell Nucleus / ultrastructure Cells, Cultured DNA, Complementary / analysis DNA, Complementary / genetics Fungal Proteins / genetics Fungal Proteins / metabolism Genes, Regulator / genetics Giant Cells / cytology Giant Cells / metabolism Intracellular Membranes / metabolism* Intracellular Membranes / ultrastructure Meiosis / physiology Microscopy, Electron Microtubules / metabolism Molecular Sequence Data Schizosaccharomyces / cytology Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / isolation & purification* Schizosaccharomyces pombe Proteins / metabolism* Spindle Apparatus / genetics Spindle Apparatus / metabolism Spindle Apparatus / ultrastructure Spores, Fungal / metabolism* Spores, Fungal / ultrastructure |
| IF | 4.573 |
| Times Cited | 36 |
| WOS Category | CELL BIOLOGY |
| Resource | |
| Yeast | CD16-1 FY12692(JZ670) FY7476(AB4) FY7325(TN187) MKD3 FY7236(SI51) YN68 |