RRC ID 903
Author Okuda Y, Sasaki D, Nogami S, Kaneko Y, Ohya Y, Anraku Y.
Title Occurrence, horizontal transfer and degeneration of VDE intein family in Saccharomycete yeasts.
Journal Yeast
Abstract VDE is a homing endonuclease gene originally discovered as an intervening element in VMA1s of Saccharomyces cerevisiae. There have been two independent subfamilies of VDE, one from S. cerevisiae strain X2180-1A and the other from Saccharomyces sp. DH1-1A in the host VMA1 gene, and they share the identity of 96.3%. In order to search the occurrence, intra/interspecies transfer and molecular degeneration of VDE, complete sequences of VMA1 in 10 strains of S. cerevisiae, eight species of saccharomycete yeasts, Candida glabrata and Kluyveromyces lactis were determined. We found that six of 10 S. cerevisiae strains contain VDEs 99.7-100% identical to that of the strain X2180-1A, one has no VDE, whereas the other three harbour VDEs 100% identical to that of the strain DH1-1A. S. carlsbergensis has two VMA1s, one being 99.8% identical to that of the strain X2180-1A with VDE 100% identical to that of the strain DH1-1A and the other containing the same VMA1 in S. pastorianus with no VDE. This and other evidence indicates that intra/interspecies transmissions of VDEs have occurred among saccharomycete yeasts. Phylogenetic analyses of VMA1 and VDE suggest that the S. cerevisiae VDEs had branched earlier than other VDEs from an ancestral VDE and had invaded into the host loci as relatively late events. The two VDEs seemed to degenerate in individual host loci, retaining their splicing capacity intact. The degeneration of the endonuclease domains was distinct and, if compared, its apparent rate was much faster than that of the protein-splicing domains.
Volume 20(7)
Pages 563-73
Published 2003-5
DOI 10.1002/yea.984
PMID 12734795
MeSH Amino Acid Substitution Base Sequence Candida glabrata / enzymology Candida glabrata / genetics DNA, Fungal / genetics Evolution, Molecular Gene Transfer, Horizontal Genes, Fungal Kluyveromyces / enzymology Kluyveromyces / genetics Models, Molecular Phylogeny Protein Structure, Tertiary Proton-Translocating ATPases / chemistry Proton-Translocating ATPases / genetics* RNA, Fungal / genetics RNA, Ribosomal / genetics Saccharomyces / enzymology Saccharomyces / genetics Saccharomyces cerevisiae / enzymology Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins / chemistry Saccharomyces cerevisiae Proteins / genetics* Saccharomycetales / enzymology Saccharomycetales / genetics* Sequence Homology, Nucleic Acid Species Specificity
IF 2.283
Times Cited 27
WOS Category MYCOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Yeast BY20282(IFO11023) BY20107(IFO1992) BY21386(IFO0211) BY21389(IFO1128) BY20544(IFO11024) BY21387(IFO0316) BY20597(IFO1267)