論文 - 詳細
| RRC ID | 36142 |
|---|---|
| 著者 | Zhu L, Mon H, Xu J, Lee JM, Kusakabe T. |
| タイトル | CRISPR/Cas9-mediated knockout of factors in non-homologous end joining pathway enhances gene targeting in silkworm cells. |
| ジャーナル | Sci Rep |
| Abstract |
Gene targeting can be achieved by precise genetic modifications through homology-directed repair (HDR) after DNA breaks introduced by genome editing tools such as CRISPR/Cas9 system. The most common form of HDR is homologous recombination (HR). Binding to the DNA breaks by HR factors is thought to compete with non-homologous end joining (NHEJ), an alternative DNA repair pathway. Here, we knocked out the factors in NHEJ by CRISPR/Cas9 system in silkworm cells, so that increased the activities of HR up to 7-fold. Also efficient HR-mediated genome editing events occurred between the chromosomal BmTUDOR-SN gene and donor DNA sequences with an EGFP gene in the middle of two homologous arms for the target gene. Utilizing the NHEJ-deficient silkworm cells, we found that homologous arms as short as 100 bp in donor DNA could be designed to perform precise genome editing. These studies should greatly accelerate investigations into genome editing of silkworm. |
| 巻・号 | 5 |
| ページ | 18103 |
| 公開日 | 2015-12-10 |
| DOI | 10.1038/srep18103 |
| PII | srep18103 |
| PMID | 26657947 |
| PMC | PMC4674802 |
| MeSH | Animals Animals, Genetically Modified Base Sequence Bombyx / cytology Bombyx / genetics* CRISPR-Cas Systems* Cells, Cultured DNA End-Joining Repair* Gene Knockout Techniques / methods* Gene Targeting / methods* Genetic Engineering / methods Genome, Insect / genetics Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Homologous Recombination Insect Proteins / genetics Insect Proteins / metabolism Microscopy, Fluorescence Molecular Sequence Data Signal Transduction / genetics |
| IF | 3.998 |
| 引用数 | 15 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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