論文 - 詳細
| RRC ID | 10928 |
|---|---|
| 著者 | Suzuki J, Yamaguchi K, Kajikawa M, Ichiyanagi K, Adachi N, Koyama H, Takeda S, Okada N. |
| タイトル | Genetic evidence that the non-homologous end-joining repair pathway is involved in LINE retrotransposition. |
| ジャーナル | PLoS Genet |
| Abstract |
Long interspersed elements (LINEs) are transposable elements that proliferate within eukaryotic genomes, having a large impact on eukaryotic genome evolution. LINEs mobilize via a process called retrotransposition. Although the role of the LINE-encoded protein(s) in retrotransposition has been extensively investigated, the participation of host-encoded factors in retrotransposition remains unclear. To address this issue, we examined retrotransposition frequencies of two structurally different LINEs--zebrafish ZfL2-2 and human L1--in knockout chicken DT40 cell lines deficient in genes involved in the non-homologous end-joining (NHEJ) repair of DNA and in human HeLa cells treated with a drug that inhibits NHEJ. Deficiencies of NHEJ proteins decreased retrotransposition frequencies of both LINEs in these cells, suggesting that NHEJ is involved in LINE retrotransposition. More precise characterization of ZfL2-2 insertions in DT40 cells permitted us to consider the possibility of dual roles for NHEJ in LINE retrotransposition, namely to ensure efficient integration of LINEs and to restrict their full-length formation. |
| 巻・号 | 5(4) |
| ページ | e1000461 |
| 公開日 | 2009-4-1 |
| DOI | 10.1371/journal.pgen.1000461 |
| PMID | 19390601 |
| PMC | PMC2666801 |
| MeSH | Animals Chickens DNA Repair* DNA Repair Enzymes / metabolism HeLa Cells Humans Long Interspersed Nucleotide Elements* Models, Genetic Mutagenesis, Insertional* Zebrafish |
| IF | 5.175 |
| 引用数 | 73 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | DT40(RCB1464) SHIP^(-) DT40(RCB1465) IP3R^(-) DT40(RCB1467) |