Reference - Detail
|Author||del Alamo D, Mlodzik M.|
|Title||Frizzled/PCP-dependent asymmetric neuralized expression determines R3/R4 fates in the Drosophila eye.|
Planar cell polarity (PCP) is a common feature in many epithelia, reflected in cellular organization within the plane of an epithelium. In the Drosophila eye, Frizzled (Fz)/PCP signaling induces cell-fate specification of the R3/R4 photoreceptors through regulation of Notch activation in R4. Except for Dl upregulation in R3, the mechanism of how Fz/PCP signaling regulates Notch in this context is not understood. We demonstrate that the E3-ubiquitin ligase Neuralized (Neur), required for Dl-N signaling, is asymmetrically expressed within the R3/R4 pair. It is required in R3, where it is also upregulated in a Fz/PCP-dependent manner. As is the case for Dl, N activity in R4 further represses neur expression, thus, reinforcing the asymmetry. We demonstrate that Neur asymmetry is instructive in correct R3/R4 specification. Our data indicate that Fz/PCP-dependent Neur expression in R3 ensures the proper directionality of Dl-N signaling during R3/R4 specification.
|MeSH||Animals Animals, Genetically Modified Cell Lineage Cell Polarity* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development* Drosophila melanogaster / metabolism Eye / embryology* Eye / metabolism Fluorescent Antibody Technique Frizzled Receptors / genetics Frizzled Receptors / metabolism* Gene Expression Regulation, Developmental Intracellular Signaling Peptides and Proteins Membrane Proteins / genetics Membrane Proteins / metabolism Photoreceptor Cells, Invertebrate Receptors, G-Protein-Coupled / genetics Receptors, G-Protein-Coupled / metabolism* Receptors, Notch / genetics Receptors, Notch / metabolism Receptors, Phencyclidine / genetics Receptors, Phencyclidine / metabolism* Signal Transduction Ubiquitin-Protein Ligases / genetics Ubiquitin-Protein Ligases / metabolism*|
|WOS Category||DEVELOPMENTAL BIOLOGY CELL BIOLOGY|