| RRC ID |
11352
|
| Author |
Natsume T, Tsutsui Y, Sutani T, Dunleavy EM, Pidoux AL, Iwasaki H, Shirahige K, Allshire RC, Yamao F.
|
| Title |
A DNA polymerase alpha accessory protein, Mcl1, is required for propagation of centromere structures in fission yeast.
|
| Journal |
PLoS One
|
| Abstract |
Specialized chromatin exists at centromeres and must be precisely transmitted during DNA replication. The mechanisms involved in the propagation of these structures remain elusive. Fission yeast centromeres are composed of two chromatin domains: the central CENP-A(Cnp1) kinetochore domain and flanking heterochromatin domains. Here we show that fission yeast Mcl1, a DNA polymerase alpha (Pol alpha) accessory protein, is critical for maintenance of centromeric chromatin. In a screen for mutants that alleviate both central domain and outer repeat silencing, we isolated several cos mutants, of which cos1 is allelic to mcl1. The mcl1-101 mutation causes reduced CENP-A(Cnp1) in the central domain and an aberrant increase in histone acetylation in both domains. These phenotypes are also observed in a mutant of swi7(+), which encodes a catalytic subunit of Pol alpha. Mcl1 forms S-phase-specific nuclear foci, which colocalize with those of PCNA and Pol alpha. These results suggest that Mcl1 and Pol alpha are required for propagation of centromere chromatin structures during DNA replication.
|
| Volume |
3(5)
|
| Pages |
e2221
|
| Published |
2008-5-21
|
| DOI |
10.1371/journal.pone.0002221
|
| PMID |
18493607
|
| PMC |
PMC2376062
|
| MeSH |
Acetylation
Centromere*
Chromatin Immunoprecipitation
Chromosomes, Fungal
Cloning, Molecular
DNA Polymerase I / metabolism*
Gene Silencing
Histone Deacetylases / genetics
Histones / metabolism
Microscopy, Fluorescence
Mutation
Myeloid Cell Leukemia Sequence 1 Protein
Proto-Oncogene Proteins c-bcl-2 / physiology*
Schizosaccharomyces / enzymology
Schizosaccharomyces / genetics
Schizosaccharomyces / metabolism
Schizosaccharomyces / ultrastructure*
|
| IF |
2.74
|
| Times Cited |
15
|
|
WOS Category
|
CELL BIOLOGY
|
| Resource |
| Yeast |
FY8452 |
