論文 - 詳細
| RRC ID | 11608 |
|---|---|
| 著者 | Yoshioka Y, Suyari O, Yamada M, Ohno K, Hayashi Y, Yamaguchi M. |
| タイトル | Complex interference in the eye developmental pathway by Drosophila NF-YA. |
| ジャーナル | Genesis |
| Abstract |
The CCAAT motif-binding factor NF-Y consists of three different subunits, NF-YA, NF-YB, and NF-YC, all of which are required for formation of the NF-Y complex and DNA-binding. NF-YA contains a DNA binding domain in its C-terminal region. We established transgenic fly lines carrying the UAS-HA-dNF-YA or UAS-dNF-YAIR and showed over-expression or knockdown with various GAL4 drivers to be lethal at various developmental stages, suggesting that dNF-YA participate in various gene regulatory pathways during Drosophila development. Expression of dNF-YA with eyeless-GAL4 mainly resulted in lethality with a headless phenotype in pharate-adults. Reduction of the eyeless gene dose enhanced the dNF-YA-induced phenotype, while reduction of the Distal-less gene dose suppressed the phenotype. On the other hand, crossing the dNF-YA over-expressing flies with Notch mutant resulted in no apparent effect on the phenotype. These results suggest that dNF-YA can disturb eye disc specification, but not eye disc growth. |
| 巻・号 | 45(1) |
| ページ | 21-31 |
| 公開日 | 2007-1-1 |
| DOI | 10.1002/dvg.20260 |
| PMID | 17216611 |
| MeSH | Amino Acid Sequence Animals Animals, Genetically Modified CCAAT-Binding Factor / genetics CCAAT-Binding Factor / metabolism* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Drosophila / embryology* Drosophila / genetics Drosophila / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism Eye / embryology* Eye / metabolism Gene Expression Regulation, Developmental Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Molecular Sequence Data Mutation Phenotype Protein Binding Protein Subunits / genetics Protein Subunits / metabolism Receptors, Notch / genetics Receptors, Notch / metabolism Sequence Homology, Amino Acid Transcription Factors / genetics Transcription Factors / metabolism |
| IF | 1.76 |
| 引用数 | 15 |
| WOS 分野 | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | |