RRC ID 11647
Author Zhao H, Zheng X, Yuan X, Wang L, Wang X, Zhong Y, Xie Z, Tully T.
Title ben Functions with scamp during synaptic transmission and long-term memory formation in Drosophila.
Journal J. Neurosci.
Abstract Genetic screens for Drosophila mutants defective in pavlovian olfactory memory have provided unique insight into the molecular basis of memory storage. Occasionally, these singular genetic lesions have been assembled into meaningful molecular pathways and neural circuitries. For the most part, however, these genes and their expression patterns in the CNS remain fragmented, demanding new clues from continued mutant screens. From a behavioral screen for long-term memory (LTM) mutants, we have identified ben (CG32594), which encodes a novel protein. Mutations of ben specifically disrupt LTM, leaving earlier memory phases intact. The role of ben appears physiological rather than developmental, because acutely induced expression of a ben(+) transgene in adults rescues the mutant's LTM defect. More interestingly, induced expression of ben(+) specifically in mushroom bodies (MBs), but not in the ellipsoid body of the central complex, is sufficient to rescue the mutant LTM defect. This suggests a role for ben in the MB during olfactory memory formation. We also provide evidence that BEN interacts genetically in both synaptic transmission and LTM formation with SCAMP, a synaptic protein known to be involved in vesicle recycling.
Volume 29(2)
Pages 414-24
Published 2009-1-14
DOI 10.1523/JNEUROSCI.5036-07.2009
PII 29/2/414
PMID 19144841
PMC PMC3045784
MeSH Analysis of Variance Animals Animals, Genetically Modified Behavior, Animal Carrier Proteins / genetics Carrier Proteins / physiology* Conditioning, Classical / physiology Drosophila / genetics Drosophila Proteins / genetics Drosophila Proteins / physiology* Gene Expression Regulation / genetics Larva Memory / physiology* Memory Disorders / genetics* Motor Activity / genetics Mushroom Bodies / metabolism Mutation / genetics Neuromuscular Junction / genetics Olfactory Pathways / physiology RNA, Messenger / metabolism Synaptic Transmission / genetics* Ubiquitin-Conjugating Enzymes / genetics Ubiquitin-Conjugating Enzymes / physiology*
IF 5.971
Times Cited 9
Drosophila w[*] P{GawB}NP4013/FM7c (DGRC#113382)