RRC ID 11683
Author Sato D, Sugimura K, Satoh D, Uemura T.
Title Crossveinless-c, the Drosophila homolog of tumor suppressor DLC1, regulates directional elongation of dendritic branches via down-regulating Rho1 activity.
Journal Genes Cells
Abstract Diverse neuronal subtypes develop distinctive morphologies of dendritic arbors that receive synaptic or sensory inputs. Dendritic arbors of many subtypes take on a polarized shape, and one underlying mechanism is unidirectionally biased elongation of dendritic branches. As reported herein, we found that Drosophila Crossveinless-c (Cv-c) was a key regulator for such directional growth. In the cv-c mutant, two subclass of multidendritic sensory neurons examined formed dorsally directed branches; however, dendritic branches had difficulty in growing along the anterior-posterior (A-P) body axis. Cv-c belongs to the family of Rho GTPase-activating proteins (RhoGAPs) and is the homolog of human tumor suppressor DLC1. The RhoGAP activity of Cv-c was required cell-autonomously for the A-P-oriented growth, and Cv-c elevated the GTPase activity of Rho1 and Cdc42 in a cell-free assay. Our analysis of genetic interactions suggested that Rho1 was the target of Cv-c in vivo. All of our results suggest that Cv-c contributes to sprouting and subsequent growth of the A-P-oriented branches through negative regulation of Rho1. We discuss a role of Cv-c in dendritic growth in response to environmental cues.
Volume 15(5)
Pages 485-500
Published 2010-5
DOI 10.1111/j.1365-2443.2010.01399.x
PMID 20384791
MeSH Alternative Splicing Animals Body Patterning / physiology Dendrites / metabolism* Dendrites / ultrastructure* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster* / anatomy & histology Drosophila melanogaster* / physiology GTPase-Activating Proteins / genetics GTPase-Activating Proteins / metabolism* Humans Phenotype Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Tumor Suppressor Proteins / genetics Tumor Suppressor Proteins / metabolism rac GTP-Binding Proteins / genetics rac GTP-Binding Proteins / metabolism rho GTP-Binding Proteins / genetics rho GTP-Binding Proteins / metabolism*
IF 1.922
Times Cited 7