Reference - Detail
| RRC ID | 11685 |
|---|---|
| Author | Seabrooke S, Qiu X, Stewart BA. |
| Title | Nonmuscle Myosin II helps regulate synaptic vesicle mobility at the Drosophila neuromuscular junction. |
| Journal | BMC Neurosci |
| Abstract |
BACKGROUND:Although the mechanistic details of the vesicle transport process from the cell body to the nerve terminal are well described, the mechanisms underlying vesicle traffic within nerve terminal boutons is relatively unknown. The actin cytoskeleton has been implicated but exactly how actin or actin-binding proteins participate in vesicle movement is not clear. RESULTS:In the present study we have identified Nonmuscle Myosin II as a candidate molecule important for synaptic vesicle traffic within Drosophila larval neuromuscular boutons. Nonmuscle Myosin II was found to be localized at the Drosophila larval neuromuscular junction; genetics and pharmacology combined with the time-lapse imaging technique FRAP were used to reveal a contribution of Nonmuscle Myosin II to synaptic vesicle movement. FRAP analysis showed that vesicle dynamics were highly dependent on the expression level of Nonmuscle Myosin II. CONCLUSION:Our results provide evidence that Nonmuscle Myosin II is present presynaptically, is important for synaptic vesicle mobility and suggests a role for Nonmuscle Myosin II in shuttling vesicles at the Drosophila neuromuscular junction. This work begins to reveal the process by which synaptic vesicles traverse within the bouton. |
| Volume | 11 |
| Pages | 37 |
| Published | 2010-3-16 |
| DOI | 10.1186/1471-2202-11-37 |
| PII | 1471-2202-11-37 |
| PMID | 20233422 |
| PMC | PMC2853426 |
| MeSH | Animals Axonal Transport / physiology* Drosophila / genetics Drosophila / metabolism* Drosophila Proteins / metabolism* Immunohistochemistry Membrane Proteins / metabolism* Motor Neurons / metabolism Motor Neurons / ultrastructure Muscle, Striated / innervation Myosin Heavy Chains / metabolism* Neuromuscular Junction / growth & development Neuromuscular Junction / metabolism* Neuromuscular Junction / ultrastructure Presynaptic Terminals / metabolism* Presynaptic Terminals / ultrastructure Staining and Labeling Synaptic Transmission / physiology Synaptic Vesicles / metabolism* Synaptic Vesicles / ultrastructure Tumor Suppressor Proteins / metabolism |
| IF | 2.811 |
| Times Cited | 26 |
| WOS Category | NEUROSCIENCES |
| Resource | |
| Drosophila | UASactinGFP (UAS-actGFP) |