Reference - Detail
| RRC ID | 11760 |
|---|---|
| Author | Ohata S, Aoki R, Kinoshita S, Yamaguchi M, Tsuruoka-Kinoshita S, Tanaka H, Wada H, Watabe S, Tsuboi T, Masai I, Okamoto H. |
| Title | Dual roles of Notch in regulation of apically restricted mitosis and apicobasal polarity of neuroepithelial cells. |
| Journal | Neuron |
| Abstract |
How the mitosis of neuroepithelial stem cells is restricted to the apical ventricular area remains unclear. In zebrafish, the mosaic eyes(rw306) (moe/epb41l5(rw306)) mutation disrupts the interaction between the putative adaptor protein Moe and the apicobasal polarity regulator Crumbs (Crb), and impairs the maintenance of neuroepithelial apicobasal polarity. While Crb interacts directly with Notch and inhibits its activity, Moe reverses this inhibition. In the moe(rw306) hindbrain, Notch activity is significantly reduced, and the number of cells that proliferate basally away from the apical area is increased. Surprisingly, activation of Notch in the moe(rw306) mutant rescues not only the basally localized proliferation but also the aberrant neuroepithelial apicobasal polarity. We present evidence that the Crb⋅Moe complex and Notch play key roles in a positive feedback loop to maintain the apicobasal polarity and the apical-high basal-low gradient of Notch activity in neuroepithelial cells, both of which are essential for their apically restricted mitosis. |
| Volume | 69(2) |
| Pages | 215-30 |
| Published | 2011-1-27 |
| DOI | 10.1016/j.neuron.2010.12.026 |
| PII | S0896-6273(10)01074-3 |
| PMID | 21262462 |
| MeSH | Animals Animals, Genetically Modified Cell Movement / physiology Cell Polarity / physiology* Embryonic Development / physiology Eye Proteins / genetics Eye Proteins / metabolism* Glycogen Synthase Kinase 3 / metabolism Glycogen Synthase Kinase 3 beta Mitosis / physiology* Motor Neurons / cytology Motor Neurons / physiology Mutation Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Neuroepithelial Cells* / cytology Neuroepithelial Cells* / physiology Proto-Oncogene Proteins c-akt / metabolism Receptors, Notch / genetics Receptors, Notch / metabolism* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Stem Cells / cytology Stem Cells / physiology Zebrafish / anatomy & histology Zebrafish / physiology Zebrafish Proteins / genetics Zebrafish Proteins / metabolism* |
| IF | 14.415 |
| Times Cited | 61 |
| WOS Category | NEUROSCIENCES |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | F1000Research |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.2 |
| Resource | |
| Zebrafish | rw306 (mosaic eyes) rw0 (Tg(CM-isl1:GFP)) Tg(vsx1:EGFP)? |