RRC ID 12065
Author Naiki T, Nagaki M, Shidoji Y, Kojima H, Imose M, Kato T, Ohishi N, Yagi K, Moriwaki H.
Title Analysis of gene expression profile induced by hepatocyte nuclear factor 4alpha in hepatoma cells using an oligonucleotide microarray.
Journal J. Biol. Chem.
Abstract Hepatocyte nuclear factor 4alpha (HNF-4alpha), a liver-specific transcription factor, plays a significant role in many liver-specific functions, including lipid, glucose, drug, and ammonia metabolism, and also in embryonal liver development. However, its functions and regulation are not yet clearly understood. In this study, we constructed an adenovirus vector carrying rat HNF-4alpha cDNA and transfected the adenovirus to human hepatoma cells, HuH-7, to enforce expression of the exogenous HNF-4alpha gene. We analyzed HNF-4alpha-induced genes using cDNA microarray technology, which included over 9000 genes. As a result, 62 genes showed a greater than 2.0-fold change in expression level after the viral transfection. Fifty-six genes were consistently induced by HNF-4alpha overexpression, and six genes were repressed. To assess HNF-4alpha function, we attempted to classify the genes, which had been classified by their encoding protein functions in a previous report. We could classify 45 genes. The rest of the HNF-4alpha-sensitive genes were unclassified (4 genes) or not identified (13 genes). Among the classified genes, almost half of the induced genes (26 of 40) were related to metabolism genes and particularly to lipid metabolism-related genes. This cDNA microarray analysis showed that HNF-4alpha is one of the central liver metabolism regulators.
Volume 277(16)
Pages 14011-9
Published 2002-4-19
DOI 10.1074/jbc.M105403200
PII M105403200
PMID 11834723
MeSH Adenoviridae / metabolism Animals Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Blotting, Northern Blotting, Western COS Cells Carcinoma, Hepatocellular / metabolism* DNA, Complementary / metabolism DNA-Binding Proteins* Down-Regulation Gene Expression Regulation* Gene Transfer Techniques Hepatocyte Nuclear Factor 4 Hepatocytes / metabolism* Humans Lipid Metabolism Liver / metabolism Microscopy, Phase-Contrast Oligonucleotide Array Sequence Analysis* Phenotype Phosphoproteins / metabolism* Rats Time Factors Transcription Factors / metabolism* Tumor Cells, Cultured Up-Regulation beta-Galactosidase / metabolism
IF 4.011
Times Cited 63
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
DNA material AxCANLacZ (RDB03211)