| RRC ID |
12117
|
| Author |
Nojima H, Rothhämel S, Shimizu T, Kim CH, Yonemura S, Marlow FL, Hibi M.
|
| Title |
Syntabulin, a motor protein linker, controls dorsal determination.
|
| Journal |
Development
|
| Abstract |
In amphibian and teleost embryos, the dorsal determinants (DDs) are believed to be initially localized to the vegetal pole and then transported to the prospective dorsal side of the embryo along a microtubule array. The DDs are known to activate the canonical Wnt pathway and thereby promote the expression of genes that induce the dorsal organizer. Here, by identifying the locus of the maternal-effect ventralized mutant tokkaebi, we show that Syntabulin, a linker of the kinesin I motor protein, is essential for dorsal determination in zebrafish. We found that syntabulin mRNA is transported to the vegetal pole during oogenesis through the Bucky ball (Buc)-mediated Balbiani body-dependent pathway, which is necessary for establishment of animal-vegetal (AV) oocyte polarity. We demonstrate that Syntabulin is translocated from the vegetal pole in a microtubule-dependent manner. Our findings suggest that Syntabulin regulates the microtubule-dependent transport of the DDs, and provide evidence for the link between AV and dorsoventral axis formation.
|
| Volume |
137(6)
|
| Pages |
923-33
|
| Published |
2010-3-1
|
| DOI |
10.1242/dev.046425
|
| PII |
dev.046425
|
| PMID |
20150281
|
| MeSH |
Animals
Animals, Genetically Modified
Body Patterning / genetics*
Cell Polarity / genetics
Cells, Cultured
Cloning, Molecular
Embryo, Nonmammalian
Female
Humans
Male
Mice
Mice, Inbred BALB C
Microtubule-Associated Proteins / genetics
Microtubule-Associated Proteins / metabolism
Microtubule-Associated Proteins / physiology*
Molecular Motor Proteins / genetics
Molecular Motor Proteins / physiology
Protein Transport
RNA, Messenger, Stored / genetics
RNA, Messenger, Stored / physiology
Zebrafish / embryology
Zebrafish / genetics
Zebrafish Proteins / genetics
Zebrafish Proteins / metabolism
Zebrafish Proteins / physiology*
|
| IF |
5.611
|
| Times Cited |
55
|
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
|
| Resource |
| Zebrafish |
Tol2 transposon system
tkk rk4 |
