RRC ID 12117
著者 Nojima H, Rothhämel S, Shimizu T, Kim CH, Yonemura S, Marlow FL, Hibi M.
タイトル Syntabulin, a motor protein linker, controls dorsal determination.
ジャーナル Development
Abstract In amphibian and teleost embryos, the dorsal determinants (DDs) are believed to be initially localized to the vegetal pole and then transported to the prospective dorsal side of the embryo along a microtubule array. The DDs are known to activate the canonical Wnt pathway and thereby promote the expression of genes that induce the dorsal organizer. Here, by identifying the locus of the maternal-effect ventralized mutant tokkaebi, we show that Syntabulin, a linker of the kinesin I motor protein, is essential for dorsal determination in zebrafish. We found that syntabulin mRNA is transported to the vegetal pole during oogenesis through the Bucky ball (Buc)-mediated Balbiani body-dependent pathway, which is necessary for establishment of animal-vegetal (AV) oocyte polarity. We demonstrate that Syntabulin is translocated from the vegetal pole in a microtubule-dependent manner. Our findings suggest that Syntabulin regulates the microtubule-dependent transport of the DDs, and provide evidence for the link between AV and dorsoventral axis formation.
巻・号 137(6)
ページ 923-33
公開日 2010-3-1
DOI 10.1242/dev.046425
PII dev.046425
PMID 20150281
MeSH Animals Animals, Genetically Modified Body Patterning / genetics* Cell Polarity / genetics Cells, Cultured Cloning, Molecular Embryo, Nonmammalian Female Humans Male Mice Mice, Inbred BALB C Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism Microtubule-Associated Proteins / physiology* Molecular Motor Proteins / genetics Molecular Motor Proteins / physiology Protein Transport RNA, Messenger, Stored / genetics RNA, Messenger, Stored / physiology Zebrafish / embryology Zebrafish / genetics Zebrafish Proteins / genetics Zebrafish Proteins / metabolism Zebrafish Proteins / physiology*
IF 5.611
引用数 55
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ゼブラフィッシュ Tol2 transposon system tkk rk4