RRC ID 12206
Author Yamashita T, Murakami T, Otani S, Kuwajima M, Shima K.
Title Leptin receptor signal transduction: OBRa and OBRb of fa type.
Journal Biochem Biophys Res Commun
Abstract We report herein the characterization of activities of signal transduction for three types of leptin receptors (OBRs) from rats, the OBRa, OBRb, and OBRb with fa mutation (OBRb-fa), by measurement of the levels of tyrosine phosphorylation of STAT3 (signal transducers and activators of transcription 3) and MAPK (mitogen-activated protein kinase), which are induced by leptin stimulation of CHO cells stably expressing the OBR (CHO-OBRb, CHO-OBRa, or CHO-OBRb-fa cells). As the result of leptin stimulation, enhanced levels of tyrosine phosphorylation of STAT3 and MAPK were detected in CHO-OBRb cells. In CHO-OBRb-fa cells, enhancement levels for both were lower than those in CHO-OBRb cells. In CHO-OBRa cells, only the phosphorylation of MAPK was detected. These data suggest that these reduced signaling activities cause obesity in fa/fa rats and that OBRa, which has been generally thought to be inactive at signaling, actually transmits signals through the MAPK pathway.
Volume 246(3)
Pages 752-9
Published 1998-5-29
DOI 10.1006/bbrc.1998.8689
PII S0006-291X(98)98689-2
PMID 9618284
MeSH Animals CHO Cells Calcium-Calmodulin-Dependent Protein Kinases / metabolism Carrier Proteins / classification Carrier Proteins / genetics Carrier Proteins / metabolism* Cricetinae DNA-Binding Proteins / metabolism Enzyme Activation Gene Expression Regulation Leptin MAP Kinase Kinase 1 Mitogen-Activated Protein Kinase Kinases* Mutation Phosphorylation Protein-Serine-Threonine Kinases / antagonists & inhibitors Protein-Tyrosine Kinases / antagonists & inhibitors Proteins / metabolism* Rats Receptors, Cell Surface* Receptors, Cytokine / classification Receptors, Cytokine / genetics Receptors, Cytokine / metabolism* Receptors, Leptin Recombinant Proteins / metabolism STAT3 Transcription Factor Signal Transduction Trans-Activators / metabolism Transcription, Genetic
IF 2.705
Times Cited 128
DNA material 465.20 Mouse JunB (RDB01298)