RRC ID 12539
Author Kashiwazaki J, Yamasaki Y, Itadani A, Teraguchi E, Maeda Y, Shimoda C, Nakamura T.
Title Endocytosis is essential for dynamic translocation of a syntaxin 1 orthologue during fission yeast meiosis.
Journal Mol. Biol. Cell
Abstract Syntaxin is a component of the target soluble N-ethylmaleimide-sensitive factor attachment protein receptor complex, which is responsible for fusion of membrane vesicles at the target membrane. The fission yeast syntaxin 1 orthologue Psy1 is essential for both vegetative growth and spore formation. During meiosis, Psy1 disappears from the plasma membrane (PM) and dramatically relocalizes on the nascent forespore membrane, which becomes the PM of the spore. Here we report the molecular details and biological significance of Psy1 relocalization. We find that, immediately after meiosis I, Psy1 is selectively internalized by endocytosis. In addition, a meiosis-specific signal induced by the transcription factor Mei4 seems to trigger this internalization. The internalization of many PM proteins is facilitated coincident with the initiation of meiosis, whereas Pma1, a P-type ATPase, persists on the PM even during the progression of meiosis II. Ergosterol on the PM is also important for the internalization of PM proteins in general during meiosis. We consider that during meiosis in Schizosaccharomyces pombe cells, the characteristics of endocytosis change, thereby facilitating internalization of Psy1 and accomplishing sporulation.
Volume 22(19)
Pages 3658-70
Published 2011-10
DOI 10.1091/mbc.E11-03-0255
PII mbc.E11-03-0255
PMID 21832151
PMC PMC3183020
MeSH Cell Membrane / metabolism Endocytosis / genetics* Gene Expression Regulation, Fungal Meiosis / genetics* Mutation Protein Transport Qa-SNARE Proteins / genetics Qa-SNARE Proteins / metabolism* Schizosaccharomyces / genetics Schizosaccharomyces / growth & development* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Spindle Apparatus / genetics Spindle Apparatus / metabolism Spores, Fungal / genetics Spores, Fungal / metabolism
IF 3.512
Times Cited 6
WOS Category CELL BIOLOGY
Resource
Yeast FY13227(TN344) FY12296 FY12710(YN68)? TN8?