RRC ID 12586
Author Helmlinger D, Marguerat S, Villén J, Gygi SP, Bähler J, Winston F.
Title The S. pombe SAGA complex controls the switch from proliferation to sexual differentiation through the opposing roles of its subunits Gcn5 and Spt8.
Journal Genes Dev.
Abstract The SAGA complex is a conserved multifunctional coactivator known to play broad roles in eukaryotic transcription. To gain new insights into its functions, we performed biochemical and genetic analyses of SAGA in the fission yeast, Schizosaccharomyces pombe. Purification of the S. pombe SAGA complex showed that its subunit composition is identical to that of Saccharomyces cerevisiae. Analysis of S. pombe SAGA mutants revealed that SAGA has two opposing roles regulating sexual differentiation. First, in nutrient-rich conditions, the SAGA histone acetyltransferase Gcn5 represses ste11(+), which encodes the master regulator of the mating pathway. In contrast, the SAGA subunit Spt8 is required for the induction of ste11(+) upon nutrient starvation. Chromatin immunoprecipitation experiments suggest that these regulatory effects are direct, as SAGA is physically associated with the ste11(+) promoter independent of nutrient levels. Genetic tests suggest that nutrient levels do cause a switch in SAGA function, as spt8Delta suppresses gcn5Delta with respect to ste11(+) derepression in rich medium, whereas the opposite relationship, gcn5Delta suppression of spt8Delta, occurs during starvation. Thus, SAGA plays distinct roles in the control of the switch from proliferation to differentiation in S. pombe through the dynamic and opposing activities of Gcn5 and Spt8.
Volume 22(22)
Pages 3184-95
Published 2008-11-15
DOI 10.1101/gad.1719908
PII 22/22/3184
PMID 19056896
PMC PMC2593614
MeSH Acetyltransferases / genetics Acetyltransferases / metabolism Acetyltransferases / physiology* Blotting, Northern Chromatin Immunoprecipitation Mass Spectrometry Oligonucleotide Array Sequence Analysis Protein Binding Reverse Transcriptase Polymerase Chain Reaction Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism Saccharomyces cerevisiae Proteins / physiology Schizosaccharomyces / genetics Schizosaccharomyces / metabolism Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Schizosaccharomyces pombe Proteins / physiology* Trans-Activators / genetics Trans-Activators / metabolism Trans-Activators / physiology* Transcription Factors / genetics Transcription Factors / metabolism Transcription Factors / physiology
IF 9.462
Times Cited 45
WOS Category GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY CELL BIOLOGY
Resource
Yeast ?