RRC ID 12655
Author Iwaki N, Karatsu K, Miyamoto M.
Title Role of guanine nucleotide exchange factors for Rho family GTPases in the regulation of cell morphology and actin cytoskeleton in fission yeast.
Journal Biochem. Biophys. Res. Commun.
Abstract Rho GTPases regulate fundamental processes including cell morphology and migration in various organisms. Guanine nucleotide exchange factor (GEF) has a crucial role in activating small GTPase by exchange GDP for GTP. In fission yeast Schizosaccharomyces pombe, six members of the Rho small GTPase family were identified and reported to be involved in cell morphology and polarized cell growth. We identified seven genes encoding Rho GEF domain from genome sequence and analyzed. Overexpressions of identified genes in cell lead to change of morphology, suggesting that all of them are involved in the regulation of cell morphology. Although all of null mutants were viable, two of seven null cells had morphology defects and five of seven displayed altered actin cytoskeleton arrangements. Most of the double mutants were viable and biochemical analysis revealed that each of GEFs bound to several small G proteins. These data suggest that identified Rho GEFs are involved in the regulation of cell morphology and share signals via small GTPase Rho family.
Volume 312(2)
Pages 414-20
Published 2003-12-12
PII S0006291X03022381
PMID 14637153
MeSH Actins Amino Acid Sequence Cell Size Cytoskeleton Gene Expression Regulation, Enzymologic / physiology* Guanine Nucleotide Exchange Factors / chemistry Guanine Nucleotide Exchange Factors / genetics* Guanine Nucleotide Exchange Factors / metabolism* Molecular Sequence Data Osmotic Pressure Recombinant Proteins / chemistry Recombinant Proteins / metabolism Schizosaccharomyces / chemistry Schizosaccharomyces / cytology* Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Sequence Homology Signal Transduction / physiology rho GTP-Binding Proteins / chemistry rho GTP-Binding Proteins / genetics* rho GTP-Binding Proteins / metabolism*
IF 2.705
Times Cited 17
Yeast cDNA libraries