論文 - 詳細
| RRC ID | 12682 |
|---|---|
| 著者 | Mitra P, Zhang Y, Rameh LE, Ivshina MP, McCollum D, Nunnari JJ, Hendricks GM, Kerr ML, Field SJ, Cantley LC, Ross AH. |
| タイトル | A novel phosphatidylinositol(3,4,5)P3 pathway in fission yeast. |
| ジャーナル | J Cell Biol |
| Abstract |
The mammalian tumor suppressor, phosphatase and tensin homologue deleted on chromosome 10 (PTEN), inhibits cell growth and survival by dephosphorylating phosphatidylinositol-(3,4,5)-trisphosphate (PI[3,4,5]P3). We have found a homologue of PTEN in the fission yeast, Schizosaccharomyces pombe (ptn1). This was an unexpected finding because yeast (S. pombe and Saccharomyces cerevisiae) lack the class I phosphoinositide 3-kinases that generate PI(3,4,5)P3 in higher eukaryotes. Indeed, PI(3,4,5)P3 has not been detected in yeast. Surprisingly, upon deletion of ptn1 in S. pombe, PI(3,4,5)P3 became detectable at levels comparable to those in mammalian cells, indicating that a pathway exists for synthesis of this lipid and that the S. pombe ptn1, like mammalian PTEN, suppresses PI(3,4,5)P3 levels. By examining various mutants, we show that synthesis of PI(3,4,5)P3 in S. pombe requires the class III phosphoinositide 3-kinase, vps34p, and the phosphatidylinositol-4-phosphate 5-kinase, its3p, but does not require the phosphatidylinositol-3-phosphate 5-kinase, fab1p. These studies suggest that a pathway for PI(3,4,5)P3 synthesis downstream of a class III phosphoinositide 3-kinase evolved before the appearance of class I phosphoinositide 3-kinases. |
| 巻・号 | 166(2) |
| ページ | 205-11 |
| 公開日 | 2004-7-19 |
| DOI | 10.1083/jcb.200404150 |
| PII | jcb.200404150 |
| PMID | 15249580 |
| PMC | PMC2172303 |
| MeSH | Evolution, Molecular Mutation Phosphatidylinositol 3-Kinases / genetics Phosphatidylinositol Phosphates / biosynthesis Phosphatidylinositol Phosphates / metabolism* Phosphoric Monoester Hydrolases / genetics* Phosphoric Monoester Hydrolases / metabolism Phosphoric Monoester Hydrolases / physiology Phosphotransferases (Alcohol Group Acceptor) / genetics Saccharomyces cerevisiae Proteins / genetics Schizosaccharomyces / cytology Schizosaccharomyces / enzymology Schizosaccharomyces / metabolism* Schizosaccharomyces / ultrastructure Schizosaccharomyces pombe Proteins* |
| IF | 8.811 |
| 引用数 | 61 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | |