| RRC ID |
1305
|
| 著者 |
Oikawa K, Kimura S, Aoki N, Atsuta Y, Takiyama Y, Nagato T, Yanai M, Kobayashi H, Sato K, Sasajima T, Tateno M.
|
| タイトル |
Neuronal calcium sensor protein visinin-like protein-3 interacts with microsomal cytochrome b5 in a Ca2+-dependent manner.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Visinin-like protein-3, which is one of the neuronal calcium sensors, has been shown to be mainly expressed in cerebellar Purkinje cells, but cellular function of this protein has not yet been elucidated. We examined the tissue distribution of murine visinin-like protein-3 transcripts using real-time reverse transcription-PCR. Visinin-like protein-3 mRNA was found to be expressed in peripheral tissues. Particularly, the expression of the transcript in the thymus was significantly higher than in other peripheral tissues. In addition, B6RVTC1 thymoma cells robustly expressed visinin-like protein-3 mRNA. To identify a target protein of visinin-like protein-3, we performed a pull-down experiment using glutathione S-transferase-tagged visinin-like protein-3 and two-dimensional electrophoresis. We demonstrated that microsomal cytochrome b(5) was a Ca(2+)-dependent binding partner of visinin-like protein-3. In a co-immunoprecipitation experiment, it was observed that hippocalcin, as well as visinin-like protein-3, could interact with cytochrome b(5). Furthermore, we confirmed that the sequence Val(114)-Tyr(127) at the C-terminal tail of cytochrome b(5) is the minimal structural requirement for binding to visinin-like protein-3. In addition, the loop His(19)-His(25) at the N terminus of visinin-like protein-3 is essential for binding to cytochrome b(5). Microsomal cytochrome b(5) was also shown to be a potential activator of cytochrome P450. The present findings raise the possibility that visinin-like protein-3 may link Ca(2+) signaling to the machinery of microsomal monooxygenase complex composed of cytochrome b(5), cytochrome P450, and some reductases. This report provides the first evidence of an interaction between visinin-like protein-3 and microsomal cytochrome b(5).
|
| 巻・号 |
279(15)
|
| ページ |
15142-52
|
| 公開日 |
2004-4-9
|
| DOI |
10.1074/jbc.M312766200
|
| PII |
S0021-9258(19)63912-8
|
| PMID |
14739275
|
| MeSH |
Amino Acid Sequence
Animals
Blotting, Western
Calcium / metabolism*
Calcium-Binding Proteins / chemistry*
Calcium-Binding Proteins / metabolism*
Cattle
Cell Line
Cell Line, Tumor
Cloning, Molecular
Cytochrome P-450 Enzyme System / metabolism
Cytochromes b5 / chemistry
Cytochromes b5 / metabolism*
Electrophoresis, Gel, Two-Dimensional
Genetic Vectors
Glutathione Transferase / metabolism
Humans
Mice
Mice, Inbred C57BL
Microsomes / metabolism
Models, Biological
Models, Molecular
Molecular Sequence Data
Mutation
Nerve Tissue Proteins / chemistry*
Nerve Tissue Proteins / metabolism*
Neurocalcin
Neurons / metabolism*
Precipitin Tests
Protein Binding
Protein Structure, Tertiary
RNA, Messenger / metabolism
Receptors, Calcium-Sensing / metabolism*
Recombinant Fusion Proteins / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Transcription, Genetic
Transfection
Tyrosine / chemistry
Valine / chemistry
|
| IF |
4.238
|
| 引用数 |
17
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
M1(RCB0169) |
