Reference - Detail
| RRC ID | 1379 |
|---|---|
| Author | Saito Y, Doi K, Yamagishi N, Ishihara K, Hatayama T. |
| Title | Screening of Hsp105alpha-binding proteins using yeast and bacterial two-hybrid systems. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Hsp105alpha is a 105-kDa stress protein, which is expressed constitutively at especially high levels in the brain compared with other tissues in mammals, and is also induced by a variety of stressors. Recently, we have shown that Hsp105alpha binds to alpha-tubulin and prevents the heat-induced disaggregation of microtubules. To further elucidate the function of Hsp105alpha, we searched for Hsp105alpha-binding proteins by screening a mouse FM3A cell library and human and mouse brain cDNA libraries using the yeast and bacterial two-hybrid systems. We showed here that Hsp105alpha interacted with several cellular proteins, such as cofilin, dynein light chain 2A, alpha-adducin, ubiquitin activating enzyme E1, phosphoglycerate kinase 1, and platelet-activating factor acethylhydrolase alpha1-subunit. The interaction was validated by the results of a pull-down assay and indirect immunofluorescence analysis. The significance of Hsp105alpha and Hsp105alpha-binding proteins in cells was discussed. |
| Volume | 314(2) |
| Pages | 396-402 |
| Published | 2004-2-6 |
| DOI | 10.1016/j.bbrc.2003.12.108 |
| PII | S0006291X03026962 |
| PMID | 14733918 |
| MeSH | Animals Bacterial Proteins / chemistry Brain / metabolism COS Cells Calmodulin-Binding Proteins / metabolism Cytoskeleton / metabolism DNA, Complementary / metabolism Fluorescent Antibody Technique, Indirect Fungal Proteins / chemistry Gene Library HSP110 Heat-Shock Proteins HSP70 Heat-Shock Proteins / metabolism* Humans Mice Microscopy, Fluorescence Microtubules / metabolism Plasmids / metabolism Protein Binding Protein Biosynthesis Recombinant Proteins / metabolism Transcription, Genetic Tubulin / metabolism Two-Hybrid System Techniques* |
| IF | 2.985 |
| Times Cited | 4 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | COS-7(RCB0539) |