Reference - Detail
| RRC ID | 15356 |
|---|---|
| Author | Gambis A, Dourlen P, Steller H, Mollereau B. |
| Title | Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila. |
| Journal | Dev Biol |
| Abstract |
We report a new two-color fluorescent imaging system to visualize the mosaic adult photoreceptor neurons (PRs) in real-time. Using this method, we examined a collection of 434 mutants and identified genes required for PR survival, planar cell polarity (PCP), patterning and differentiation. We could track the progression of PR degeneration in living flies. By introducing the expression of p35, a caspase inhibitor, we found mutations that specifically activate caspase-dependent death. Moreover, we showed that grh is required in R3 for correct PCP establishment. The "Tomato/GFP-FLP/FRT" method allows high-throughput, rapid and precise identification of survival and developmental pathways in living adult PRs at single-cell resolution. |
| Volume | 351(1) |
| Pages | 128-34 |
| Published | 2011-3-1 |
| DOI | 10.1016/j.ydbio.2010.12.040 |
| PII | S0012-1606(10)01296-0 |
| PMID | 21215264 |
| PMC | PMC3051417 |
| MeSH | Animals Apoptosis* Cell Polarity Cell Survival DNA-Binding Proteins / physiology Drosophila / physiology* Drosophila Proteins / physiology Fluorescence Mutation Photoreceptor Cells, Invertebrate / physiology* Transcription Factors / physiology |
| IF | 2.896 |
| Times Cited | 26 |
| WOS Category | DEVELOPMENTAL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | FRT-lethal |