論文 - 詳細
| RRC ID | 15356 |
|---|---|
| 著者 | Gambis A, Dourlen P, Steller H, Mollereau B. |
| タイトル | Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila. |
| ジャーナル | Dev Biol |
| Abstract |
We report a new two-color fluorescent imaging system to visualize the mosaic adult photoreceptor neurons (PRs) in real-time. Using this method, we examined a collection of 434 mutants and identified genes required for PR survival, planar cell polarity (PCP), patterning and differentiation. We could track the progression of PR degeneration in living flies. By introducing the expression of p35, a caspase inhibitor, we found mutations that specifically activate caspase-dependent death. Moreover, we showed that grh is required in R3 for correct PCP establishment. The "Tomato/GFP-FLP/FRT" method allows high-throughput, rapid and precise identification of survival and developmental pathways in living adult PRs at single-cell resolution. |
| 巻・号 | 351(1) |
| ページ | 128-34 |
| 公開日 | 2011-3-1 |
| DOI | 10.1016/j.ydbio.2010.12.040 |
| PII | S0012-1606(10)01296-0 |
| PMID | 21215264 |
| PMC | PMC3051417 |
| MeSH | Animals Apoptosis* Cell Polarity Cell Survival DNA-Binding Proteins / physiology Drosophila / physiology* Drosophila Proteins / physiology Fluorescence Mutation Photoreceptor Cells, Invertebrate / physiology* Transcription Factors / physiology |
| IF | 2.896 |
| 引用数 | 26 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | FRT-lethal |