RRC ID 17460
Author Chen YS, Wu HC, Liu CH, Chen HC, Yanagida F.
Title Isolation and characterization of lactic acid bacteria from jiang-sun (fermented bamboo shoots), a traditional fermented food in Taiwan.
Journal J. Sci. Food Agric.
Abstract BACKGROUND:In this study, lactic acid bacteria (LAB) were isolated, characterized and identified from jiang-sun (fermented bamboo shoots; a traditional fermented food in Taiwan). Samples were collected at seven time intervals from a fixed fermenting bucket during the fermentation process of jiang-sun and its initial ingredients, dochi (fermented soybeans) and bamboo shoots.
RESULTS:A total of 57 LAB cultures were isolated; 42 cultures were isolated from jiang-sun samples and 15 cultures were isolated from dochi and bamboo shoots. These isolates were characterized phenotypically and then divided into three groups (A-C) by restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Alteration of microbial populations during the fermentation process was observed. While various LAB were found in the dochi and bamboo shoots, it was mostly replaced by Lactobacillus plantarum after 1 day of fermentation. Furthermore, the antibacterial activities of the isolates were determined, and one Enterococcus faecium strain showed inhibitory activity against all the indicator strains.
CONCLUSION:Results suggest that L. plantarum is the main LAB present during the fermentation of jiang-sun. To our knowledge, this is the first report describing the distribution and varieties of LAB that exist in the jiang-sun fermentation process.
Volume 90(12)
Pages 1977-82
Published 2010-9
DOI 10.1002/jsfa.4034
PMID 20698013
MeSH Anti-Bacterial Agents / isolation & purification DNA, Ribosomal Enterococcus faecium / genetics Enterococcus faecium / isolation & purification* Fermentation Food Microbiology* Lactobacillaceae / classification Lactobacillaceae / genetics Lactobacillaceae / isolation & purification Lactobacillus plantarum / genetics Lactobacillus plantarum / isolation & purification* Plant Shoots / microbiology RNA, Ribosomal, 16S Sasa / microbiology* Seeds / microbiology Soybeans / microbiology* Taiwan
IF 2.379
Times Cited 15
WOS Category AGRICULTURE, MULTIDISCIPLINARY FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED
Resource
General Microbes JCM 1157