| RRC ID |
17580
|
| Author |
Nakase M, Tsukamoto Y, Hosomi A, Matsuda T, Miyamoto M, Takegawa K.
|
| Title |
CUE domain-containing protein Vps901 is required for vacuolar protein transport in Schizosaccharomyces pombe.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
The functions of two Schizosaccharomyces pombe Vps9-like genes, SPBC4F6.10/vps901(+) and SPBC29A10.11c/vps902(+), were characterized. Genomic sequence analysis predicted that Vps901p contains a VPS9 domain, whereas cDNA analyses revealed that Vps901p contains a CUE domain (coupling of ubiquitin to ER degradation) in its C-terminal region. Deletion of vps901(+) resulted in mis-sorting and secretion of S. pombe vacuolar carboxypeptidase Cpy1p, whereas deletion of vps902(+) had no effect, suggesting that only Vps901p functions in vacuolar protein transport in S. pombe. Deletion of vps901(+) further produced pleiotropic phenotypes, including vacuolar homotypic fusion and endocytosis defects. Heterologous expression of the budding yeast VPS9 gene corrected the CPY mis-sorting defect in vps901Δ cells. These findings suggest that the VPS9 domain of Vps901p is required for vacuolar protein trafficking in S. pombe.
|
| Volume |
76(4)
|
| Pages |
652-9
|
| Published |
2012-1-1
|
| DOI |
10.1271/bbb.110609
|
| PII |
JST.JSTAGE/bbb/110609
|
| PMID |
22484924
|
| MeSH |
Amino Acid Sequence
Carboxypeptidases / metabolism
Endocytosis
Genetic Complementation Test
Guanine Nucleotide Exchange Factors / genetics*
Guanine Nucleotide Exchange Factors / metabolism
Membrane Fusion
Microscopy, Fluorescence
Molecular Sequence Data
Protein Isoforms / genetics
Protein Isoforms / metabolism
Protein Structure, Tertiary
Protein Transport / physiology*
Schizosaccharomyces / genetics*
Schizosaccharomyces / metabolism
Schizosaccharomyces pombe Proteins / genetics*
Schizosaccharomyces pombe Proteins / metabolism
Sequence Deletion
Sequence Homology, Amino Acid
Vacuoles / physiology*
|
| IF |
1.516
|
| Times Cited |
3
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CHEMISTRY, APPLIED
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Yeast |
|
