Reference - Detail
| RRC ID | 17622 |
|---|---|
| Author | Takeuchi T, Adachi Y, Nagayama T, Furihata M. |
| Title | Nedd4L modulates the transcription of metalloproteinase-1 and -13 genes to increase the invasive activity of gallbladder cancer. |
| Journal | Int J Exp Pathol |
| Abstract |
The aim of this study was to examine whether Nedd4L (neural precursor cell expressed, developmentally down-regulated 4-like) participated in gallbladder carcinogenesis. We first immunohistochemically examined the expression of Nedd4L in various gallbladder tissue specimens. Weak immunoreactivity to Nedd4L-specific antibody was observed in normal or dysplastic epithelial cells. Cancer cells in non-invasive regions exhibited little immunoreactivity, whereas strong immunostaining was found in cytoplasm of many invasive cancers, especially at cancer invasive front with desmoplastic reaction. Notably, siRNA-mediated silencing of the Nedd4L gene significantly decreased the Matrigel-invasion activity and collagen invasion activity of cultured gallbladder cancer cells, without affecting the cell growth. The subtractive mRNA hybridization followed by RT-PCR and immunoblotting revealed that down-regulation of Nedd4L significantly decreased the expression of collagenases, matrix metalloproteinase (MMP)-1 and -13, in gallbladder cancer cells. Finally, immunohistochemical staining showed that many Nedd4L-expressing invasive gallbladder cancer cells co-expressed MMP-1 and MMP-13. These results indicated that over-expression of Nedd4L might lead to gallbladder cancer invasion by regulating the transcription of the MMP-1 and MMP-13 genes. |
| Volume | 92(2) |
| Pages | 79-86 |
| Published | 2011-4-1 |
| DOI | 10.1111/j.1365-2613.2010.00740.x |
| PMID | 21039987 |
| PMC | PMC3081510 |
| MeSH | Adenocarcinoma / genetics* Adenocarcinoma / metabolism Adenocarcinoma / pathology Aged Cell Line, Tumor Down-Regulation Endosomal Sorting Complexes Required for Transport / genetics* Endosomal Sorting Complexes Required for Transport / metabolism Female Gallbladder Neoplasms / genetics* Gallbladder Neoplasms / metabolism Gallbladder Neoplasms / pathology Gene Expression Regulation, Neoplastic* Gene Silencing Humans Immunohistochemistry Male Matrix Metalloproteinase 1 / genetics* Matrix Metalloproteinase 1 / metabolism Matrix Metalloproteinase 13 / genetics* Matrix Metalloproteinase 13 / metabolism Middle Aged Nedd4 Ubiquitin Protein Ligases Neoplasm Invasiveness RNA, Small Interfering / genetics Transcription, Genetic Transcriptional Activation Ubiquitin-Protein Ligases / genetics* Ubiquitin-Protein Ligases / metabolism |
| IF | 1.672 |
| Times Cited | 21 |
| WOS Category | PATHOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Human and Animal Cells | TGBC1TKB(RCB1129) |